FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29057055
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAA3913821.3469431089971093No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGG2836220.9760865304484574No Hit
TGGAGGAGGAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTTA2481390.8539716086162207No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAAT1883500.6482074663106774No Hit
TGGATAATGAATTATAATTAATATTATTAATTAATATTTATTATTAATAAT1272620.43797280901316393No Hit
TGGATGTTTAAATTAAATTATTTTTATTGTTATATGATTGAGATCGGAAGA1269740.43698165557383567No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG1096380.37731972493427157No Hit
TGGAGTAATTTAGGTTGGTTTTTATTTATTTTAAATTTTTTTTTGTATGAA890980.3066312122821807No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG679160.2337332534215873No Hit
TGGTTTTAGTGTTTTGTAATGTTATTGTGTGTATATTTTTTAGATGAAAAT613340.21108126752693965No Hit
TGGGAAAAAAGGTGGGAGAAGTTTTGAGATCGGAAGAGCACACGTCTGAAC557860.1919877977998803Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG555730.19125475723537708No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA511030.17587122989580328No Hit
TGGGTTTATAATATTTGGGGGTAGTTAAAGTGAATTGTATTTGATATTTGG467750.16097639626589824No Hit
TGGTTGTTAGGTGTTTAATGGGGTTTAGTAGGGTGGGGTTATTTTTGTTAA455510.1567639941487532No Hit
TGGTGGGGATAGTGATGATTATGGTAGTGGAGGTGAAATATGTTTGTGTGT446390.15362534159088043No Hit
TGGAAAAAAAGAATTATTTGGATATATAGGTATGGTTTGAGTTATGATATT395500.13611152265775042No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC382040.13147925693088994No Hit
TGGTGGGGTTGAAGAAGGTGGTGTTGAGGTTGTGGTTTGTTAGTAGTATAG368220.1267230970241134No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATAG329560.1134182387031308No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG305160.10502096650882205No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTA304280.10471811406902728No Hit
TGGGGTTTTTTTTGTTTTTTTTTTTGAGATCGGAAGAGCACACGTCTGAAC300860.103541119359825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG297690.1024501622755644No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG297310.10231938508565304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)