FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29057055
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACAATAAACCATAACCAATACTACCAATCAATACTCATCATTAATAAT2904710.9996573981774823No Hit
CAAAAAAAAAAACCCCATTCTATACCAACACCTATTCTAATTTTTCAATCA2623490.9028753946330762No Hit
CAAAATAATAAATTACAATATAAAAAATTATTCCAAAACCTAATAAAATAA2493020.8579740789285081No Hit
CAAAATAATCCAAATCAATTTCTATCTACTTCAAATTCCTCCCTATACAAA1856930.6390633875318747No Hit
CAAATATCAAAAAAATACCTTAAATAACCTCTAAAACTCAAAAATAAAAAA1292840.4449315321184476No Hit
CAATCATATAACAATAAAAATAATTTAATTTAAACATCCAAGATCGGAAGA1268650.4366065315290899No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1261260.43406325933581363No Hit
CAAACTCTACCATCTTAACAAACCCTATTCTTAAATAAATATAAATATAAT909270.3129257249229146No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA898800.30932246919035666No Hit
CAAACCCATAACACTTAAAAATAACTAAAATAAACTATATCCAACATCTAA597250.20554388598569262No Hit
CAAAAAAAAAAAACCCCATTCTATACCAACACCTATTCTAATTTTTCAATC556870.19164708880511117No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG553310.19042191302594155No Hit
CAAAACTTCTCCCACCTTTTTTCCCAAGATCGGAAGAGCGTCGTGTAGGGA544220.18729358498306178Illumina Single End PCR Primer 1 (100% over 25bp)
CAACTCCAACATCTCACAATACTATCACATACACACCCCCCAAACAAAAAT470530.1619331346552498No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA441690.15200783424197667No Hit
CAAATCCATCATCCACAACCTTAACAATAAACAAAATATTCAAAAAATAAA408520.14059236216471355No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG380730.1310284197761955No Hit
CAATAAAAATAACAATAATTATAATAACAAAAATAAAATATACTCATATAT378590.13029193770669464No Hit
CAAAACAAAAACAAATTAAACAATCTACCCTCCCTTAACAAAAAACTACTC352820.12142317932770544No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACA350960.12078305939813927No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG307050.1056714109533812No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG305080.10499343446884069No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACCG291910.10046097238691257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)