FastQCFastQC Report
Fri 10 May 2024
EGAF00003472157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3263995
Total Bases461.7 Mbp
Sequences flagged as poor quality0
Sequence length6-348
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTTGTCCATGAGCTTCCAGCACTTCTCCACCATCTTCTTGTCCACCG57930.1774818895249533No Hit
CAGCAACCTCACCCTTCCTTGTTCCTCCACCTCATTCCAGGCCTAAGATC54110.16577844022432633No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC52690.16142794336388383No Hit
CCAGTTGCCGGATCTCCAAGTACTCAAAGTCCATCTCCTCCGCCAGCGCG51760.15857867429331232No Hit
CAACCTGATGGCGGGGCCGGGGCGGGAGCTGCTCTCACGGCTGCGGCTGT50210.1538298925090265No Hit
TCCCTCATTTCCTCCTGCAGCTGCAAAGCTGTCCCATCAAGGAAGACAGC50000.15318650917051035No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG49670.152175478209985No Hit
CCCTGCAGGTCAGTACTGTACCGAGGATGTGGACGAGTGCCAGCTGATGC48660.1490811107247407No Hit
GCCAACACTACACACTGTGCTATTCACCAGAGAGTCACAATATTTGACAG48050.14721223531286046No Hit
CCTTGCAAATGTTCACTGACAGCAGCACGGTGGAAAGCATCTCGCTCCAG47420.14528208529731204No Hit
GCCAAGTGCCTGGACGGACCCAACACTTACACCTGTGTGTGCACGGAAGG47140.14442424084595717No Hit
CAACTGGAGATTTACCTGACAGCGAATCATAACATGGGTCACTTTAGACT47080.14424041703495258No Hit
CAGTTTCCTTCAAGATCCTCAAGAGAGCTTGGTTGGGAGCTTCTCCACTG46900.1436889456019387No Hit
AACCCTGGGGTTGAAGACTGGGCTTGTCCCACCATGGGGCAAGGGCCTGA46770.1432906606780954No Hit
CAAACGGATCTACACGATGGGTTCAGTACCTTTAATTTCACATCGGCAGA46630.14286173845241798No Hit
CACGGACAGCACGGTTGTAGCGTATGCAGTCAACACGCAGCAGGCTGTCA46610.14280046384874978No Hit
CCGTCCTCTCCCAGACCCGTGTCACAACGGGGGCTCCTGCACAGACGGCA46370.1420651686047313No Hit
CCAACTGTAGCCCTCTGTAGTAGGCAGCTTTCCTCCTTTCTCAGAGAAGG45950.14077840192769905No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA45730.14010438128734878No Hit
CATCCAGGTTGATGGTGCAGAAGCGTCCAGCAAAGCCCTCAGGACAGAGG45300.13878697730848238No Hit
CTGCAGGAATCAGAAGGTCAGGGCTGTCCTTTCTGCCGATGTGAAATTAA45190.13844996698830728No Hit
CTGCCGACCAACGAACTGGTCCCTTTGTTCTTCCCTCTCCACAGGCAAAG44560.13651981697275886No Hit
GACTCACCTGCATCTCCAGGCAGGTCCACAGTCACAGGGAGGGACCAGGA44330.1358151590305745No Hit
GACTATTCTTGGTTTCACAGCTGATACCCAACTCTTGTGCAACTCATGCC43420.13302716456367122No Hit
CCTGTTCTCGGCATTTGCCTTAAGAGGACCCCCCACAAAAGTCTTCTCAT43280.13259824233799378No Hit
CAGCAAGCGCAAGCGGGACGAGTGGCTGGCACGCTGGAAAAGGGAGGTGA43000.1317403978866389No Hit
GTCTGACGGTTCACCTTCAGCACATGCAGCCTGGCCTCATACTTGATCCT42930.1315259367738002No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT42590.13048426851144074No Hit
GTGTGTGTGCACAGGAGGGTGTGTAGGATGTGACACTCACCGGGGGGTGC42390.1298715224747587No Hit
GGCAGGCACGCCCATGGCGACCAGGTAGGCCAGGGTGGAGAGGGGATCCA41650.12760436213903514No Hit
CCGTCATAGTTCTTGCAGGCCCACACAAAGCCACCCGAAGACTTGAGGAC41550.12729798912069412No Hit
CTCTTCCTGTCCAGGGATCACTGGAACTTGGGGCAGATACTGGTACTTTG41510.1271754399133577No Hit
GAGGGACCAGGGTTGGAAATAGAATGCGGGAAGGAGCAGCTTGGCTAAAC41380.12677715498951436No Hit
GCAGCCATGGGTGGCGCCTCCCCCTACTGCCTGCCCAGGGCTCTGTCCAG41350.12668524308401208No Hit
CTCGGGGCTGGCCCTTGTTCCTGTCGGGGTGCGAGGGCTCCTGAGCCCAC41320.12659333117850977No Hit
CAGGAGGTGTTTGGAAAGATAACATCAACATGGCTCTAGTTGTCGACACC41310.12656269387667568No Hit
CCTGGAGCCAGTAAAAGAAAATCACGGTGGGATGAAACACCAGCTAGTCA41290.12650141927300745No Hit
CCCAGGCACTATAAGACCGACTTCGACAAGAATAAGATCTGGTATGAGCA41150.12607249704733003No Hit
CCACATCCTCCCTTCCCCTGGCTGCTCTCAACATGCGAGTGCGGCGCCGC40980.1255516629161503No Hit
GGGATCCACCTCTGGCCTCACAGGCTGTGTCTGTGCCTGCAGGACGGAGA40640.12450999465379084No Hit
AGAATCAGGATCCACAGAGCCCCTGGGGGTGGAACACTTGCCTCCATTTT40610.12441808274828853No Hit
CAGATGGGTGGAAGCACTCCAGTTCTGACCCCTGGAAAGACACCAATTGG40510.1241117097299475No Hit
CTGACCCTTCCCGCTGCTGTCTAGAACTGCTTTCTGGAGTGTGCGTACCA40410.12380533671160648No Hit
GCCCAGCACCCGCCTGAGCCTCTCCCTGTTGCCCAGATCCTGATCCGGAA40400.12377469940977237No Hit
GCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCAGGTGG40360.12365215020243597No Hit
CCCAGTGGGAAGTTGGGACCAAGCACAAACTCCATGTCTGGTGGGGTACA40300.12346832639143136No Hit
TGTGGATGGCCCCGAAGCAAGCCTGATGGAACAGGATAGAACCAACCATG40250.12331513988226084No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA40240.12328450258042675No Hit
CAAGCTCTGTGCAGGTGCCACCCTCCTGGCAGCAGAGCTCCCAGGGTTTA40230.12325386527859265No Hit
TGGGCTCATGAAGGACGCCTTCCAGCCGCACCACCACCACCACCACCACC40130.12294749226025162No Hit
GGGACACCGATGGGCCTGCATCTGACCTGTTGTGCTCACTGCTTAGGACC39960.12242665812907189No Hit
GGAACTCATTGATCTTGTCCAGGCAGCGGCCATTGTGCAGGCAGGGGCTG39890.12221219701623318No Hit
ACTTCTGGATGAGCTCTCTCAGGCAGTGGGCTTCCATTCTGGAGCTTTGT39830.12202837320522857No Hit
GTGGCGCCTTCGCATGAAGAGGCCGATCCCCAGGGCCACCACCAGCAGCA39670.12153817637588293No Hit
TCCATTTCAGTGCACCATAAGATGTCCTCTTTCTCATTCATGAAGACAGG39630.12141562716854652No Hit
CTGGATGGCATACTGGAAGCAGCTGTGCGCAAAACCTGAGATGGACTGCA39440.12083351843369858No Hit
CCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTG39320.12046587081168936No Hit
CAAACCACCCTGGGTGGTTAAAGGTCAGCCCACTTACCAGATATGCCCCC39230.12019013509518245No Hit
ACCGAAGATTGCAGCCTTCTTGCCTGGGGACCATGCCCCTGTCTGTGTGC38850.11902591762548656No Hit
CCCTGCTGACATCCCTGTAGTGCGCCTCCCCACCCCAAACTTGCACTGGA38770.11878081921081374No Hit
CTCCAGGGAAATCGTGCCAGCAGGCTGACCCGTGCGCCTCCAACCCCTGC38350.11749405253378145No Hit
GCGAAGGCGCCACATCGTTCGGAAGCGCACGCTGCGGAGGCTGCTGCAGG38300.11734086602461094No Hit
GACGGAGAGCATGAAGATGGAAAGTGGCAGTCCCCTGCGCCCAGGCCTAG38140.11685066919526531No Hit
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA37780.11574772632923765No Hit
CCAGAAGCAGAATAAGAGTTGACAGACTCTGTCTGAGGGTGATGTGGCTG37650.11534944140539431No Hit
TACTTGGCTAACACTTTGGTGTGAGTATTGATGACATTCAAAGCTTCCTT37560.11507370568888738No Hit
GTGTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATT37510.11492051917971688No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT37500.11488988187788277No Hit
CCCAGGAAGTGGTGCCAGACTCACATTCAGTTCTAACATCCGGATGCAAC37480.11482860727421457No Hit
AGGGCCAAAAAGGGTTGTTGTGAACCTACCGTGAACTCTGGGATGTATCC37470.11479796997238047No Hit
TCCTTAGATGTCTCCTTGCTGGCATCAGACTCAGAGCCCCTGAAGTGTTT37440.11470605806687816No Hit
CGCGTGGGCGGCGGGGTACCGTTCCTGGAGAGCTGGGCCTTGCCCTGGGA37260.11415458663386432No Hit
GAGTTTGCTACACGGAGGGGTAACTCATTTACCTATGTAGATCCAATTCC37090.11363375250268458No Hit
CCTCTGTCCATCAAGACTAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGT37060.11354184059718228No Hit
GCAGCAGCAGAACCTGCAGCCAGCAAACATCCAGCAGCAGCAAAGCCTGC36950.11320483027700717No Hit
TGATGGGGATCAGTCGCTTCTGATGGGCACCTGCAAGCCAAGGGGTGCCA36930.11314355567333895No Hit
GTCTTGCCCTCAGGCCTGCCGGCTCCCCCCAGTATCATCAAGGCCATGGG36880.11299036916416845No Hit
GCTTAGCAGTGTATAGAGATCTCGTCCGAAACTCCCAAGATGATTATGAT36820.11280654535316384No Hit
GCTGTGGCTCCCTCACCCATCCATTAAAGGGTCCTTACCATCGTTTTCAC36820.11280654535316384No Hit
AGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGATACTCACCTGCAG36500.11182615169447258No Hit
CCCTACTGCTGGCCAGTACTGAGTACCAGCCAAGAGCCGTGTGCCTGTCC36500.11182615169447258No Hit
CGAGACGCACTCGTTGACGTCGATCTCGCATCGGGGGCCCGTGTAGCCCT36490.11179551439263846No Hit
GAGAAACGCGCAGCCCACTCACCGCTGAAGCCCTCCCGGCAGGTGCACAC36460.11170360248713615No Hit
CTCATCCTGGTGTGGGAAGGAATCCCGCTGTCTCTGGCTGACAAACTCTA36370.11142786677062924No Hit
GGCTCCAGGAGTCGGTGTTTCACCAATAGAATCTCCACCTCGATCTGTTC36330.11130531756329283No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC36250.11106021914862002No Hit
CTGTCCTAGGGCTCCCGGGGCCGGCTGCGGGGTGGCTTGGGCTGGGAGTC36180.1108457580357813No Hit
CCAGGGGTCAGAACTGGAGTGCTTCCACCCATCTGACTAGCTGGTGTTTC35900.10998791358442644No Hit
GAGTGGTGCTCTCTCTGCAGTGTGAAGCTGAACGAGCACTTCCTGAACAC35870.10989600167892413No Hit
CATCAATGTTCTCGCTGCAGTCCTCACCAGTCCAGCCGTTGACACACACG35640.10919134373673979No Hit
GCCTGGGCTCAGCGCGGTGGAAGGCGGCGTGAAGCGGCGGCTCGTGCTGG35620.10913006913307159No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT35570.1089768826239011No Hit
GAGATCACTCACCCATCGCATACCTCAGGGCAGATCAATTCCGCACAGAC35560.10894624532206698No Hit
CCCCACAAGTACCACTTACAAAAGACCAGAGCCATGGCTCGCTACGCATG35510.10879305881289646No Hit
TGTAAAGTGGTATGCCTTATCCAGAATGGAAGTTTCAAGGAAGCTTTGAA35390.10842541119088724No Hit
TCCATTCATTGAAACCTCAGCCAAGACCAGACAGGTATGGTACAGCTTTC35370.10836413658721904No Hit
GCCGATCTCAGAGGCCGTGTCTGTACTCTCATTGCTGGGGGTGGGTGAGG35310.10818031277621443No Hit
GAGGTAAATGTGGATGACTGCCTGATGCGGCCTTGTGCTAACGGTGCCAC35270.10805776356887802No Hit
CCACGACCTCCTTCTCCACTGGGCTGCTGGACCCCTGGCTGCCTTGGATT35220.10790457705970752No Hit
GACCCCAAGGCGGGCATCATGATCAAAAGTGTGAAACGCGCCTACCATCG35210.1078739397578734No Hit
ACAGCGAACACAACTACTGCCGCCTTATCCTCTAAATGGGGAGCATACCC35210.1078739397578734No Hit
GAGAGGCTCACCCTGCTGCCCCACACGCCCCACCCGCCTGGGCGCGGCAC35110.10756756673953238No Hit
GGCCACACTCGTTGACATCCTGCCGGCAGGTGGGGCCATGGAAGCTGGGT35090.10750629213586418No Hit
CTTCGGTAGTCCATCTGGGCCTGGGAGGAACACAGGGGCAAAGGAGTCCT34970.10713864451385496No Hit
CAGGGTTGTCCAGGCTAATTTGGTGGCTGCCTTTCTGGGCCCAGTGGGCA34910.10695482070285033No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG34810.10664844768450933No Hit
CAGCCAGAAGGCCGAAGGGCCGGCCTCAACGGCACCTCTCCTGGGTGGGT34800.10661781038267522No Hit
CTAGGACCAACCAGATTGGACCATGACAGCCATTCTGTATGTTGTGGACG34780.10655653577900702No Hit
CTCCACAGCACCCAAGCTCCCCAGCCAGGCCACCCACCCCTTTGAAAGAG34770.10652589847717292No Hit
GAGATCGGCAGTGCTTTCAACTCGCCACTGCGCTCGCCTATCCGCTCAGC34640.10612761355332959No Hit
GAGGTCAATGAGTGCAACAGCAACCCCTGCGTCCACGGGGCCTGCCGGGA34620.10606633894966139No Hit
TAACAACGGGCACAGGAATACTCACTTCTTGCGACGGCGGCCATACAGCT34560.10588251513865678No Hit
CTTGTGACCTCTCTTCCCAGAGGGTGGGAAGGGTTCCTCGCCCTCCATCA34430.10548423021481344No Hit
CAGCTGTATGACCGTGGGACCAGTGGAGTTCTTGGGATCTCTGGGGCCAT34350.10523913180014062No Hit
GGGAACCTGGCGGCCCACGTGCAGGGGTGGCAGAAAGGAAAAGCGGGGGG34170.10468766036712679No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC34140.10459574846162448No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC34080.10441192465061988No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA33970.10407491433044475No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT33950.10401363972677655No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC33930.10395236512310835No Hit
CCCATGGGAACAGTATTGGACCCTTACCCAGGGGGTCATCAAGTGTGGTG33890.10382981591577194No Hit
GCCAGATAGCTGTGAAACAGGCACTGGTCTTGCCAGGATTGAGGCCACCC33750.10340089369009449No Hit
CGTGGGGAGGCAGGACCACAGCTGGCCCACACCCTGCAGCCGTGACTCAC33740.1033702563882604No Hit
GGGGAGTGGGGCACAGTCCAGCACCTACCGCGGGTAGGCATACAGCAGCT33660.10312515797358758No Hit
ACGGCGTTGACGTCGGCGTGTGAGTTGATGAGGTCCTCCAGCATGCCCTC33630.10303324606808527No Hit
CCATGCAGTCGGCGTCAACCTCACCCTGGGGCGGTGTGGGGGCCATGGCA33590.10291069686074886No Hit
CCTCCCTGCCAATCCACTGCCATGGCTCAGTCTGCTTCTCTTCCTTCTCC33530.10272687304974425No Hit
GCCCGCAGCCCGCCTGACCGGAGGCCAGATGTGCCGCCCACACTTGTTGC33440.10245113733323734No Hit
GGGGTGAAGACCATTTTGAAAGTGCCGGCCCGGTCTGCCACAAAGTCTGT33350.10217540161673042No Hit
ACAGTCATCGTGACAGAACGCACCGCAGCCTTGGCACATGATCATGGCTT33160.10159329288188249No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA33160.10159329288188249No Hit
CAACTGATGCAAACTCTTGCACAAATGCTGAAAGCTGTACCATACCTGTC33150.10156265558004837No Hit
CGGGAGAGGCCAGATCAGGCAACTGGAGAAATCACCCACCTTGAACTTCT32990.10107245875070274No Hit
CTGCCTGGCTCGGCTCTCCACTCAGGAAGCTCCGGCCCAGGTGGCCGCTG32950.10094990954336634No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCGTGGCC32790.10045971271402071No Hit
GCAGTGTGGTCCCCCTTGCAGGTACAAGTGCGACTGTGACCCTGGGTGGA32780.1004290754121866No Hit
GGTGCCTACCATGGTCAATGGGGTAGACCTTCAGCCCATCCAGCTCAAAG32690.10015333969567969No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)