FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003472157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3263995
Sequences flagged as poor quality0
Sequence length6-348
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTTGTCCATGAGCTTCCAGCACTTCTCCACCATCTTCTTGTCCACCG57640.17659340777176435No Hit
CAGCAACCTCACCCTTCCTTGTTCCTCCACCTCATTCCAGGCCTAAGATC53920.16519633148947838No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC52590.1611215703455428No Hit
CCAGTTGCCGGATCTCCAAGTACTCAAAGTCCATCTCCTCCGCCAGCGCG50700.1553311202988975No Hit
CAACCTGATGGCGGGGCCGGGGCGGGAGCTGCTCTCACGGCTGCGGCTGT49530.15174655598430759No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG49340.15116444724945963No Hit
TCCCTCATTTCCTCCTGCAGCTGCAAAGCTGTCCCATCAAGGAAGACAGC49090.15039851470360707No Hit
CCCTGCAGGTCAGTACTGTACCGAGGATGTGGACGAGTGCCAGCTGATGC48440.14840709008439046No Hit
GCCAACACTACACACTGTGCTATTCACCAGAGAGTCACAATATTTGACAG47500.14552718371198484No Hit
CCTTGCAAATGTTCACTGACAGCAGCACGGTGGAAAGCATCTCGCTCCAG47110.1443323289404549No Hit
GCCAAGTGCCTGGACGGACCCAACACTTACACCTGTGTGTGCACGGAAGG47050.14414850512945027No Hit
CAGTTTCCTTCAAGATCCTCAAGAGAGCTTGGTTGGGAGCTTCTCCACTG46530.14255536543407696No Hit
CACGGACAGCACGGTTGTAGCGTATGCAGTCAACACGCAGCAGGCTGTCA46520.14252472813224284No Hit
AACCCTGGGGTTGAAGACTGGGCTTGTCCCACCATGGGGCAAGGGCCTGA46500.14246345352857465No Hit
CAACTGGAGATTTACCTGACAGCGAATCATAACATGGGTCACTTTAGACT46490.14243281622674053No Hit
CAAACGGATCTACACGATGGGTTCAGTACCTTTAATTTCACATCGGCAGA46180.14148305986988338No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA45500.13939972334516443No Hit
CCGTCCTCTCCCAGACCCGTGTCACAACGGGGGCTCCTGCACAGACGGCA45470.13930781143966214No Hit
CTGCAGGAATCAGAAGGTCAGGGCTGTCCTTTCTGCCGATGTGAAATTAA44870.137469573329616No Hit
CATCCAGGTTGATGGTGCAGAAGCGTCCAGCAAAGCCCTCAGGACAGAGG44800.1372551122167773No Hit
CCAACTGTAGCCCTCTGTAGTAGGCAGCTTTCCTCCTTTCTCAGAGAAGG44640.13676491538743166No Hit
CTGCCGACCAACGAACTGGTCCCTTTGTTCTTCCCTCTCCACAGGCAAAG44330.1358151590305745No Hit
GACTCACCTGCATCTCCAGGCAGGTCCACAGTCACAGGGAGGGACCAGGA44270.13563133521956988No Hit
CCTGTTCTCGGCATTTGCCTTAAGAGGACCCCCCACAAAAGTCTTCTCAT42880.1313727502646297No Hit
CAGCAAGCGCAAGCGGGACGAGTGGCTGGCACGCTGGAAAAGGGAGGTGA42780.13106637724628867No Hit
GTCTGACGGTTCACCTTCAGCACATGCAGCCTGGCCTCATACTTGATCCT42550.1303617193041043No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT42420.12996343438026098No Hit
GTGTGTGTGCACAGGAGGGTGTGTAGGATGTGACACTCACCGGGGGGTGC42230.12938132564541305No Hit
GACTATTCTTGGTTTCACAGCTGATACCCAACTCTTGTGCAACTCATGCC41640.12757372483720103No Hit
CCGTCATAGTTCTTGCAGGCCCACACAAAGCCACCCGAAGACTTGAGGAC41330.12662396848034388No Hit
GCAGCCATGGGTGGCGCCTCCCCCTACTGCCTGCCCAGGGCTCTGTCCAG41170.12613377165099823No Hit
GAGGGACCAGGGTTGGAAATAGAATGCGGGAAGGAGCAGCTTGGCTAAAC41170.12613377165099823No Hit
CTCGGGGCTGGCCCTTGTTCCTGTCGGGGTGCGAGGGCTCCTGAGCCCAC41130.12601122244366184No Hit
CCTGGAGCCAGTAAAAGAAAATCACGGTGGGATGAAACACCAGCTAGTCA41120.12598058514182775No Hit
GGCAGGCACGCCCATGGCGACCAGGTAGGCCAGGGTGGAGAGGGGATCCA41060.12579676133082313No Hit
CCCAGGCACTATAAGACCGACTTCGACAAGAATAAGATCTGGTATGAGCA41040.1257354867271549No Hit
CTCTTCCTGTCCAGGGATCACTGGAACTTGGGGCAGATACTGGTACTTTG40710.12472445576662955No Hit
CCACATCCTCCCTTCCCCTGGCTGCTCTCAACATGCGAGTGCGGCGCCGC40690.12466318116296134No Hit
CAGGAGGTGTTTGGAAAGATAACATCAACATGGCTCTAGTTGTCGACACC40590.12435680814462031No Hit
GGGATCCACCTCTGGCCTCACAGGCTGTGTCTGTGCCTGCAGGACGGAGA40430.1238666113152747No Hit
GCCCAGCACCCGCCTGAGCCTCTCCCTGTTGCCCAGATCCTGATCCGGAA40350.12362151290060187No Hit
AGAATCAGGATCCACAGAGCCCCTGGGGGTGGAACACTTGCCTCCATTTT40270.12337641448592906No Hit
CAGATGGGTGGAAGCACTCCAGTTCTGACCCCTGGAAAGACACCAATTGG40230.12325386527859265No Hit
TGTGGATGGCCCCGAAGCAAGCCTGATGGAACAGGATAGAACCAACCATG40150.12300876686391982No Hit
CCCAGTGGGAAGTTGGGACCAAGCACAAACTCCATGTCTGGTGGGGTACA40150.12300876686391982No Hit
GCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCAGGTGG40130.12294749226025162No Hit
TGGGCTCATGAAGGACGCCTTCCAGCCGCACCACCACCACCACCACCACC39900.12224283431806728No Hit
CAAGCTCTGTGCAGGTGCCACCCTCCTGGCAGCAGAGCTCCCAGGGTTTA39740.12175263748872163No Hit
GGAACTCATTGATCTTGTCCAGGCAGCGGCCATTGTGCAGGCAGGGGCTG39710.12166072558321933No Hit
CTGACCCTTCCCGCTGCTGTCTAGAACTGCTTTCTGGAGTGTGCGTACCA39670.12153817637588293No Hit
ACTTCTGGATGAGCTCTCTCAGGCAGTGGGCTTCCATTCTGGAGCTTTGT39570.12123180335754191No Hit
GTGGCGCCTTCGCATGAAGAGGCCGATCCCCAGGGCCACCACCAGCAGCA39550.1211705287538737No Hit
CTGGATGGCATACTGGAAGCAGCTGTGCGCAAAACCTGAGATGGACTGCA39240.12022077239701653No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA39230.12019013509518245No Hit
CAAACCACCCTGGGTGGTTAAAGGTCAGCCCACTTACCAGATATGCCCCC39040.1196080263603345No Hit
TCCATTTCAGTGCACCATAAGATGTCCTCTTTCTCATTCATGAAGACAGG38850.11902591762548656No Hit
CCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTG38780.11881145651264785No Hit
CCCTGCTGACATCCCTGTAGTGCGCCTCCCCACCCCAAACTTGCACTGGA38630.11835189698513632No Hit
CTCCAGGGAAATCGTGCCAGCAGGCTGACCCGTGCGCCTCCAACCCCTGC38040.11654429617692429No Hit
GCGAAGGCGCCACATCGTTCGGAAGCGCACGCTGCGGAGGCTGCTGCAGG37970.11632983506408556No Hit
ACCGAAGATTGCAGCCTTCTTGCCTGGGGACCATGCCCCTGTCTGTGTGC37900.11611537395124687No Hit
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA37540.11501243108521919No Hit
AGGGCCAAAAAGGGTTGTTGTGAACCTACCGTGAACTCTGGGATGTATCC37370.11449159695403946No Hit
GTGTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATT37210.11400140012469383No Hit
CGCGTGGGCGGCGGGGTACCGTTCCTGGAGAGCTGGGCCTTGCCCTGGGA37140.1137869390118551No Hit
TCCTTAGATGTCTCCTTGCTGGCATCAGACTCAGAGCCCCTGAAGTGTTT37040.11348056599351408No Hit
TACTTGGCTAACACTTTGGTGTGAGTATTGATGACATTCAAAGCTTCCTT36990.11332737948434358No Hit
GTCTTGCCCTCAGGCCTGCCGGCTCCCCCCAGTATCATCAAGGCCATGGG36810.11277590805132973No Hit
CCAGAAGCAGAATAAGAGTTGACAGACTCTGTCTGAGGGTGATGTGGCTG36600.1121325247128136No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT36540.11194870090180899No Hit
GCTGTGGCTCCCTCACCCATCCATTAAAGGGTCCTTACCATCGTTTTCAC36530.11191806359997487No Hit
CGAGACGCACTCGTTGACGTCGATCTCGCATCGGGGGCCCGTGTAGCCCT36390.11148914137429745No Hit
GCTTAGCAGTGTATAGAGATCTCGTCCGAAACTCCCAAGATGATTATGAT36380.11145850407246335No Hit
CCCTACTGCTGGCCAGTACTGAGTACCAGCCAAGAGCCGTGTGCCTGTCC36370.11142786677062924No Hit
TGATGGGGATCAGTCGCTTCTGATGGGCACCTGCAAGCCAAGGGGTGCCA36340.11133595486512693No Hit
GAGAAACGCGCAGCCCACTCACCGCTGAAGCCCTCCCGGCAGGTGCACAC36320.11127468026145874No Hit
CTCATCCTGGTGTGGGAAGGAATCCCGCTGTCTCTGGCTGACAAACTCTA36160.1107844834321131No Hit
CTGTCCTAGGGCTCCCGGGGCCGGCTGCGGGGTGGCTTGGGCTGGGAGTC35910.11001855088626056No Hit
GCAGCAGCAGAACCTGCAGCCAGCAAACATCCAGCAGCAGCAAAGCCTGC35820.10974281516975364No Hit
AGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGATACTCACCTGCAG35770.10958962866058311No Hit
GAGTGGTGCTCTCTCTGCAGTGTGAAGCTGAACGAGCACTTCCTGAACAC35730.1094670794532467No Hit
GAGTTTGCTACACGGAGGGGTAACTCATTTACCTATGTAGATCCAATTCC35560.10894624532206698No Hit
CCCCACAAGTACCACTTACAAAAGACCAGAGCCATGGCTCGCTACGCATG35450.10860923500189185No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC35390.10842541119088724No Hit
GCCTGGGCTCAGCGCGGTGGAAGGCGGCGTGAAGCGGCGGCTCGTGCTGG35220.10790457705970752No Hit
GCCGATCTCAGAGGCCGTGTCTGTACTCTCATTGCTGGGGGTGGGTGAGG35210.1078739397578734No Hit
CCAGGGGTCAGAACTGGAGTGCTTCCACCCATCTGACTAGCTGGTGTTTC35170.107751390550537No Hit
GACGGAGAGCATGAAGATGGAAAGTGGCAGTCCCCTGCGCCCAGGCCTAG34950.10707736991018675No Hit
GGCCACACTCGTTGACATCCTGCCGGCAGGTGGGGCCATGGAAGCTGGGT34920.10698545800468444No Hit
CCACGACCTCCTTCTCCACTGGGCTGCTGGACCCCTGGCTGCCTTGGATT34890.10689354609918214No Hit
ACAGCGAACACAACTACTGCCGCCTTATCCTCTAAATGGGGAGCATACCC34880.10686290879734803No Hit
TGTAAAGTGGTATGCCTTATCCAGAATGGAAGTTTCAAGGAAGCTTTGAA34860.10680163419367983No Hit
GAGAGGCTCACCCTGCTGCCCCACACGCCCCACCCGCCTGGGCGCGGCAC34860.10680163419367983No Hit
GACCCCAAGGCGGGCATCATGATCAAAAGTGTGAAACGCGCCTACCATCG34850.10677099689184572No Hit
GAGGTAAATGTGGATGACTGCCTGATGCGGCCTTGTGCTAACGGTGCCAC34800.10661781038267522No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG34780.10655653577900702No Hit
CAGGGTTGTCCAGGCTAATTTGGTGGCTGCCTTTCTGGGCCCAGTGGGCA34780.10655653577900702No Hit
TCCATTCATTGAAACCTCAGCCAAGACCAGACAGGTATGGTACAGCTTTC34720.10637271196800241No Hit
CTTCGGTAGTCCATCTGGGCCTGGGAGGAACACAGGGGCAAAGGAGTCCT34700.1063114373643342No Hit
CTCCACAGCACCCAAGCTCCCCAGCCAGGCCACCCACCCCTTTGAAAGAG34610.10603570164782727No Hit
GAGATCACTCACCCATCGCATACCTCAGGGCAGATCAATTCCGCACAGAC34570.10591315244049086No Hit
CTAGGACCAACCAGATTGGACCATGACAGCCATTCTGTATGTTGTGGACG34560.10588251513865678No Hit
GAGATCGGCAGTGCTTTCAACTCGCCACTGCGCTCGCCTATCCGCTCAGC34510.10572932862948625No Hit
TAACAACGGGCACAGGAATACTCACTTCTTGCGACGGCGGCCATACAGCT34450.10554550481848164No Hit
CATCAATGTTCTCGCTGCAGTCCTCACCAGTCCAGCCGTTGACACACACG34390.10536168100747703No Hit
GAGGTCAATGAGTGCAACAGCAACCCCTGCGTCCACGGGGCCTGCCGGGA34340.10520849449830653No Hit
CAGCTGTATGACCGTGGGACCAGTGGAGTTCTTGGGATCTCTGGGGCCAT34200.1047795722726291No Hit
CTTGTGACCTCTCTTCCCAGAGGGTGGGAAGGGTTCCTCGCCCTCCATCA34100.10447319925428807No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC34020.10422810083961526No Hit
GGGAACCTGGCGGCCCACGTGCAGGGGTGGCAGAAAGGAAAAGCGGGGGG34010.10419746353778116No Hit
CCTCTGTCCATCAAGACTAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGT33850.10370726670843552No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT33810.1035847175010991No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC33780.1034928055955968No Hit
CCCATGGGAACAGTATTGGACCCTTACCCAGGGGGTCATCAAGTGTGGTG33730.1033396190864263No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC33700.10324770718092399No Hit
GCCAGATAGCTGTGAAACAGGCACTGGTCTTGCCAGGATTGAGGCCACCC33670.1031557952754217No Hit
GGGGAGTGGGGCACAGTCCAGCACCTACCGCGGGTAGGCATACAGCAGCT33550.10278814765341247No Hit
CGTGGGGAGGCAGGACCACAGCTGGCCCACACCCTGCAGCCGTGACTCAC33540.10275751035157836No Hit
ACGGCGTTGACGTCGGCGTGTGAGTTGATGAGGTCCTCCAGCATGCCCTC33460.10251241193690554No Hit
CCATGCAGTCGGCGTCAACCTCACCCTGGGGCGGTGTGGGGGCCATGGCA33380.10226731352223273No Hit
GGCTCCAGGAGTCGGTGTTTCACCAATAGAATCTCCACCTCGATCTGTTCGAGGAGTCTCAGCCCATCCACTTCC33300.10202221510755992No Hit
CAGCCAGAAGGCCGAAGGGCCGGCCTCAACGGCACCTCTCCTGGGTGGGT33210.10174647939105298No Hit
GGGGTGAAGACCATTTTGAAAGTGCCGGCCCGGTCTGCCACAAAGTCTGT33200.1017158420892189No Hit
ACAGTCATCGTGACAGAACGCACCGCAGCCTTGGCACATGATCATGGCTT33100.10140946907087788No Hit
GCCCGCAGCCCGCCTGACCGGAGGCCAGATGTGCCGCCCACACTTGTTGC33010.10113373335437095No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT32980.10104182144886865No Hit
CAACTGATGCAAACTCTTGCACAAATGCTGAAAGCTGTACCATACCTGTC32900.10079672303419582No Hit
CGGGAGAGGCCAGATCAGGCAACTGGAGAAATCACCCACCTTGAACTTCT32830.10058226192135711No Hit
GCAGTGTGGTCCCCCTTGCAGGTACAAGTGCGACTGTGACCCTGGGTGGA32690.10015333969567969No Hit
CCTCCCTGCCAATCCACTGCCATGGCTCAGTCTGCTTCTCTTCCTTCTCC32680.10012270239384558No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCGTGGCC32670.10009206509201146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)