FastQCFastQC Report
Fri 10 May 2024
EGAF00003472158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903172
Total Bases419.7 Mbp
Sequences flagged as poor quality0
Sequence length5-389
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACACTACACACTGTGCTATTCACCAGAGAGTCACAATATTTGACAG43130.14856164223132493No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG40930.14098372400946274No Hit
CTCATCCTGGTGTGGGAAGGAATCCCGCTGTCTCTGGCTGACAAACTCTA39750.13691920423591852No Hit
TCCCTCATTTCCTCCTGCAGCTGCAAAGCTGTCCCATCAAGGAAGACAGC39090.13464582876935985No Hit
CCTTGCAAATGTTCACTGACAGCAGCACGGTGGAAAGCATCTCGCTCCAG36960.12730902612728423No Hit
CTGACCCTTCCCGCTGCTGTCTAGAACTGCTTTCTGGAGTGTGCGTACCA36670.1263101187253115No Hit
CCCAGGAAGTGGTGCCAGACTCACATTCAGTTCTAACATCCGGATGCAAC36370.12527676624051212No Hit
CCAACTGTAGCCCTCTGTAGTAGGCAGCTTTCCTCCTTTCTCAGAGAAGG35850.12348562193352651No Hit
CAGGAGGTGTTTGGAAAGATAACATCAACATGGCTCTAGTTGTCGACACC35420.12200448337198072No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC35260.12145336204675437No Hit
CCAGTTGCCGGATCTCCAAGTACTCAAAGTCCATCTCCTCCGCCAGCGCG35240.12138447188110109No Hit
CCGTCCTCTCCCAGACCCGTGTCACAACGGGGGCTCCTGCACAGACGGCA35170.12114335630131456No Hit
CACCTTGTCCATGAGCTTCCAGCACTTCTCCACCATCTTCTTGTCCACCG35090.12086779563870138No Hit
GACTATTCTTGGTTTCACAGCTGATACCCAACTCTTGTGCAACTCATGCC34970.12045445464478163No Hit
CAACCTGATGGCGGGGCCGGGGCGGGAGCTGCTCTCACGGCTGCGGCTGT34880.12014444889934184No Hit
CAAGCTCTGTGCAGGTGCCACCCTCCTGGCAGCAGAGCTCCCAGGGTTTA34530.1189388710004092No Hit
CAGCAACCTCACCCTTCCTTGTTCCTCCACCTCATTCCAGGCCTAAGATC34420.11855997508931611No Hit
CCTCCCTGCCAATCCACTGCCATGGCTCAGTCTGCTTCTCTTCCTTCTCC34360.11835330459235624No Hit
CAGATGGGTGGAAGCACTCCAGTTCTGACCCCTGGAAAGACACCAATTGG34250.11797440868126312No Hit
CAACTGGAGATTTACCTGACAGCGAATCATAACATGGGTCACTTTAGACT34240.11793996359843646No Hit
CAGGAGAGGCCAGGGGAGGGGAGCTGAAGGACACGGCCCTCAGCAGGGCA34010.1171477266934236No Hit
CCCTACTGCTGGCCAGTACTGAGTACCAGCCAAGAGCCGTGTGCCTGTCC33600.11573547829753111No Hit
ACCGAAGATTGCAGCCTTCTTGCCTGGGGACCATGCCCCTGTCTGTGTGC33180.11428878481881198No Hit
CCGTTTGATCCTAGAGGGAGTGGCAGCCTGTTGAGGCAAGGAGCAGAGGG32510.11198096426942668No Hit
CTCTTCCTGTCCAGGGATCACTGGAACTTGGGGCAGATACTGGTACTTTG32480.11187762902094675No Hit
CCTCTGTCCATCAAGACTAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGT32470.11184318393812011No Hit
CCTCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGT32430.11170540360681351No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG32060.11043093554222759No Hit
CACGGACAGCACGGTTGTAGCGTATGCAGTCAACACGCAGCAGGCTGTCA31930.1099831494654812No Hit
CCCAGGGGATATGCTGAACTAAGTTGCCACAGGACCTGCAGCCTGCCCAC31900.10987981421700127No Hit
GACCCCAAGGCGGGCATCATGATCAAAAGTGTGAAACGCGCCTACCATCG31800.1095353633887348No Hit
GCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCAGGTGG31740.10932869289177494No Hit
GCCCGCAGCCCGCCTGACCGGAGGCCAGATGTGCCGCCCACACTTGTTGC31710.10922535764329498No Hit
GCCAAGTGCCTGGACGGACCCAACACTTACACCTGTGTGTGCACGGAAGG31520.10857090106958871No Hit
CAGGGAAGAGGCTGACCCGGCCCCTCTTGCGGCAGGCTTCACCGGCCAGA31480.10843312073828212No Hit
CTGCCGACCAACGAACTGGTCCCTTTGTTCTTCCCTCTCCACAGGCAAAG31300.10781310924740249No Hit
CCCTGCAGGTCAGTACTGTACCGAGGATGTGGACGAGTGCCAGCTGATGC31010.10681420184542975No Hit
CCCCACAAGTACCACTTACAAAAGACCAGAGCCATGGCTCGCTACGCATG30990.10674531167977647No Hit
CAGCAAGCGCAAGCGGGACGAGTGGCTGGCACGCTGGAAAAGGGAGGTGA30960.10664197643129653No Hit
GTGTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATT30650.1055741788636705No Hit
CAAACGGATCTACACGATGGGTTCAGTACCTTTAATTTCACATCGGCAGA30430.10481638704148429No Hit
CCCAGTGGGAAGTTGGGACCAAGCACAAACTCCATGTCTGGTGGGGTACA30390.10467860671017769No Hit
GACAGCAATGGCAGTTACTGTCAACAGGTGGACATTGAAAAGCTGAAAAT30290.10433415588191125No Hit
CAAACCACCCTGGGTGGTTAAAGGTCAGCCCACTTACCAGATATGCCCCC29880.10292190748601873No Hit
GAGGTCAATGAGTGCAACAGCAACCCCTGCGTCCACGGGGCCTGCCGGGA29840.10278412715471216No Hit
TCCTTAGATGTCTCCTTGCTGGCATCAGACTCAGAGCCCCTGAAGTGTTT29810.10268079190623222No Hit
CTTCGGTAGTCCATCTGGGCCTGGGAGGAACACAGGGGCAAAGGAGTCCT29810.10268079190623222No Hit
GGTACTTGTGGGGATTCTGACATGCTGTCATCGTGGGCTGGTGGAAGAAC29650.10212967058100587No Hit
GAGTTTGCTACACGGAGGGGTAACTCATTTACCTATGTAGATCCAATTCC29510.10164743942143284No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA29490.10157854925577955No Hit
CTCGGGGCTGGCCCTTGTTCCTGTCGGGGTGCGAGGGCTCCTGAGCCCAC29440.10140632384164631No Hit
TCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGTAATTGATGGAGAAA29430.10137187875881967No Hit
CCCAAGTCACAGCTCCCCACCACAAAAACGCAAATGCTCCCCTCTTCCAC29330.1010274279305532No Hit
AGCCTGCCTCAGGAAACTGTGGATCAGGAACCCAAGGATCAGAAGAGGAA29300.10092409268207325No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT29270.10082075743359332No Hit
CAGCACATGTTGGATGGGTGGCCATCCAGCGGACATCTCCTTCCTGTTGT29190.10054519677098014No Hit
CCCTGCTGACATCCCTGTAGTGCGCCTCCCCACCCCAAACTTGCACTGGA29140.10037297135684692No Hit
CGTCGGTGTTGTACATGCCCATGCCCACGCCGCCTGCGGGGAAGTTGTAC29050.10006296561140711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)