FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003472172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5597986
Sequences flagged as poor quality0
Sequence length8-320
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC91430.16332659638662905No Hit
CTTACCAGCCTGGTGGATCCAGAAAGGGGAGCCCAGGTAGCTGTGAACAG89080.15912865805666537No Hit
GGAAAGGTACCCGGGTTGTGCTGGCATCTGGCTGCAGTGTGGCTGTTCTG87960.15712793851217205No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT81830.1461775717195434No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG78470.14017541308606343No Hit
CCTCCCTGCCAATCCACTGCCATGGCTCAGTCTGCTTCTCTTCCTTCTCC75600.13504856925329933No Hit
GGGAAATGCTTGATAAAACCCACTGTTCCCAGGACGTTTGTGGAAAACAA70350.12567019638848687No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG69800.12468770018360174No Hit
TCCATTCATTGAAACCTCAGCCAAGACCAGACAGGTATGGTACAGCTTTC67510.12059694325780737No Hit
ACCGAAGATTGCAGCCTTCTTGCCTGGGGACCATGCCCCTGTCTGTGTGC67140.1199359912654301No Hit
TTCTCCCTTTGCCTACTTCTCCCTCTAGTACAGAATACGCCATCAATAAA67040.11975735559181463No Hit
GCCAACAGGGAGAAGCCCCGTGCTTGCCCCCTGAGTTCCCAGAGCATGCC65170.11641686849520524No Hit
GGAGGCCACAACAGCCTCACCTGCAGCAGTTGTTGTTTCCGCACATGAGC64980.11607746071533584No Hit
ATGGCCTGGTCCAAGCCAGTGTCCTGACTGACCTCAGACTTCTCAAAGCA64660.11550582655976632No Hit
CAGGTGGCCTTGCTAATTCCTGGAGAGGTCAAGGTGACTTTTTGTTTTTT63740.11386237836250394No Hit
TACCTACACTGTCCACAAAGAGGACTCGCTGAGTCCGAGTCTGGTTTAGG62480.11161156887494895No Hit
CAACTGATGCAAACTCTTGCACAAATGCTGAAAGCTGTACCATACCTGTC62260.11121857039299492No Hit
GCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCAGGTGG61250.1094143500894786No Hit
CTGTCCTAGGGCTCCCGGGGCCGGCTGCGGGGTGGCTTGGGCTGGGAGTC60430.10794953756583171No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG60360.10782449259430088No Hit
GGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCGCGAGATGTCCCT59790.10680626925469268No Hit
TGTTTACAGAAAGAGAGATTCTATGAAAGCCGGTGCAGGCCAGTGACACC57560.10282269373306757No Hit
ATTACACCGTCAAGGATGACCGGGACAGCCCTGTGTTTTGGTAACTGAAT57480.10267978519417519No Hit
ACCAGGCAGGGTTGGTGGTGGGCACAGGGGAGGGAAGCACACTCACCGGG56510.10094701916010507No Hit
ATTTTTATGCCTACAGATATCATGGTGGAAGAGTTTGTGGAAGGGGGTCC56480.10089342845802043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)