FastQCFastQC Report
Wed 8 May 2024
EGAF00003472172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5597986
Total Bases783.1 Mbp
Sequences flagged as poor quality0
Sequence length8-320
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCCTTCCCGCTGCTGTCTAGAACTGCTTTCTGGAGTGTGCGTACCA112030.20012554515141698No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC93030.16618476716447664No Hit
CTTACCAGCCTGGTGGATCCAGAAAGGGGAGCCCAGGTAGCTGTGAACAG89300.1595216565386194No Hit
GGAAAGGTACCCGGGTTGTGCTGGCATCTGGCTGCAGTGTGGCTGTTCTG88110.15739589202259527No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT82020.1465169794994128No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG78730.14063986583746368No Hit
CCTCCCTGCCAATCCACTGCCATGGCTCAGTCTGCTTCTCTTCCTTCTCC76590.13681706242209252No Hit
GGGAAATGCTTGATAAAACCCACTGTTCCCAGGACGTTTGTGGAAAACAA70520.12597387703363316No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG70070.12517001650236353No Hit
TTCTCCCTTTGCCTACTTCTCCCTCTAGTACAGAATACGCCATCAATAAA68790.12288347988008545No Hit
TCCATTCATTGAAACCTCAGCCAAGACCAGACAGGTATGGTACAGCTTTC68230.12188312010783878No Hit
ACCGAAGATTGCAGCCTTCTTGCCTGGGGACCATGCCCCTGTCTGTGTGC67690.12091848747031522No Hit
CAGGTGGCCTTGCTAATTCCTGGAGAGGTCAAGGTGACTTTTTGTTTTTT65830.11759586394106737No Hit
GCCAACAGGGAGAAGCCCCGTGCTTGCCCCCTGAGTTCCCAGAGCATGCC65480.11697063908341322No Hit
GGAGGCCACAACAGCCTCACCTGCAGCAGTTGTTGTTTCCGCACATGAGC65200.11647045919728988No Hit
ATGGCCTGGTCCAAGCCAGTGTCCTGACTGACCTCAGACTTCTCAAAGCA64830.11580950720491262No Hit
CAACTGATGCAAACTCTTGCACAAATGCTGAAAGCTGTACCATACCTGTC63140.1127905643208111No Hit
TACCTACACTGTCCACAAAGAGGACTCGCTGAGTCCGAGTCTGGTTTAGG62930.11241542940621858No Hit
GCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCAGGTGG61440.109753757869348No Hit
CTGTCCTAGGGCTCCCGGGGCCGGCTGCGGGGTGGCTTGGGCTGGGAGTC60740.10850330815403969No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG60510.1080924461047241No Hit
GGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCGCGAGATGTCCCT60190.10752081194915458No Hit
ATTACACCGTCAAGGATGACCGGGACAGCCCTGTGTTTTGGTAACTGAAT57710.10309064724349079No Hit
TGTTTACAGAAAGAGAGATTCTATGAAAGCCGGTGCAGGCCAGTGACACC57670.10301919297404458No Hit
CCAGATCCGCACGAAGGTCCAAGTCCAAGTCCTCGTCGGTCTCCAGATCT57510.10273337589625983No Hit
ACCAGGCAGGGTTGGTGGTGGGCACAGGGGAGGGAAGCACACTCACCGGG56970.10176874325873626No Hit
ATTTTTATGCCTACAGATATCATGGTGGAAGAGTTTGTGGAAGGGGGTCC56600.101107791266359No Hit
CCTGCCTTCCAGGTTCTATTTCACCAATTGGCATGGAACCAACGACAATG56430.10080411062121271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)