FastQCFastQC Report
Wed 8 May 2024
EGAF00003472173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417364
Total Bases337.1 Mbp
Sequences flagged as poor quality0
Sequence length8-311
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCCTTCCCGCTGCTGTCTAGAACTGCTTTCTGGAGTGTGCGTACCA42310.17502535820008902No Hit
GACTATTCTTGGTTTCACAGCTGATACCCAACTCTTGTGCAACTCATGCC37300.15430030396746208No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG36860.1524801395238781No Hit
CCAGTTGCCGGATCTCCAAGTACTCAAAGTCCATCTCCTCCGCCAGCGCG31290.1294385123630533No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC29930.12381254953743003No Hit
AGGATCCCATATTTTTGAGCCTGGGGTTGCTCCAGGAGTCTCGCTTCCCT29760.12310930418422711No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC29020.12004811852910856No Hit
GTGTGTGTGCACAGGAGGGTGTGTAGGATGTGACACTCACCGGGGGGTGC27740.11475309469322784No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT27660.11442215570348528No Hit
TCCATTTCAGTGCACCATAAGATGTCCTCTTTCTCATTCATGAAGACAGG27640.11433942095604964No Hit
TCCCTCATTCTCTTGCCAGAGCAGGGTTGAGCTTACCTGGAGAGAGGCTG27600.11417395146117837No Hit
CTTACCAGCCTGGTGGATCCAGAAAGGGGAGCCCAGGTAGCTGTGAACAG27340.11309839974451509No Hit
CCACATCCTCCCTTCCCCTGGCTGCTCTCAACATGCGAGTGCGGCGCCGC27050.11189874590669838No Hit
ACCGAAGATTGCAGCCTTCTTGCCTGGGGACCATGCCCCTGTCTGTGTGC26310.10883756025157983No Hit
CAGCAAGCGCAAGCGGGACGAGTGGCTGGCACGCTGGAAAAGGGAGGTGA26260.10863072338299073No Hit
CCAACCATCCAATCGGACAAGCTTTCAGAACCTTATTGAAGGATTTGAAG25690.10627278308107507No Hit
CCAAACAAAGCACAGAGTCCAGCAGACCTGGTGGGCAAAGAACATGTTAT25200.104245781768902No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC25150.1040389449003129No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT24980.10333569954710999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)