FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003472173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417364
Sequences flagged as poor quality0
Sequence length8-311
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG36390.15053587295914062No Hit
CCAGTTGCCGGATCTCCAAGTACTCAAAGTCCATCTCCTCCGCCAGCGCG30940.12799065428292966No Hit
AGGATCCCATATTTTTGAGCCTGGGGTTGCTCCAGGAGTCTCGCTTCCCT29670.12273699782076675No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC29590.1224060588310242No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT27550.11396711459258928No Hit
GTGTGTGTGCACAGGAGGGTGTGTAGGATGTGACACTCACCGGGGGGTGC27430.11347070610797548No Hit
CTTACCAGCCTGGTGGATCCAGAAAGGGGAGCCCAGGTAGCTGTGAACAG27190.11247788913874783No Hit
CCACATCCTCCCTTCCCCTGGCTGCTCTCAACATGCGAGTGCGGCGCCGC26660.11028541833170347No Hit
TCCATTTCAGTGCACCATAAGATGTCCTCTTTCTCATTCATGAAGACAGG26410.109251233988758No Hit
ACCGAAGATTGCAGCCTTCTTGCCTGGGGACCATGCCCCTGTCTGTGTGC26070.10784474328235218No Hit
CAGCAAGCGCAAGCGGGACGAGTGGCTGGCACGCTGGAAAAGGGAGGTGA26030.1076792737874809No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC25470.10536270085928308No Hit
CCAACCATCCAATCGGACAAGCTTTCAGAACCTTATTGAAGGATTTGAAG25430.10519723136441181No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT24590.10172237197211507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)