FastQCFastQC Report
Wed 8 May 2024
EGAF00003472174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8838518
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length8-343
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCAAGGCTTCAGCTCTGACAGCAGAGGGTGGAAGCTGCCTCATCTC132500.14991201013563588No Hit
CAGAGGAGTTGGTGGGTGTGAGTGCCCCTGTCCCTGCACTTCGGGTGGCT126430.14304434295432786No Hit
CTCTCAACAGGAACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTG118000.13350654487550967No Hit
GATGTTATCCGTGCGCAGGATCTGCTTCTTGAGGTGGCTCATGAGGTCTC103650.11727079132497101No Hit
CTAGGACCAACCAGATTGGACCATGACAGCCATTCTGTATGTTGTGGACG101370.11469117333924081No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT97000.10974690553325794No Hit
GTCTTGCCCTCAGGCCTGCCGGCTCCCCCCAGTATCATCAAGGCCATGGG94070.10643187013931522No Hit
CTTCCAGAATGCATTGAACGAATCCCATGGATTGCTCCTGAGTGTGTTGA93510.10579827975685516No Hit
TCCTTAGATGTCTCCTTGCTGGCATCAGACTCAGAGCCCCTGAAGTGTTT90630.10253981493277492No Hit
GGATTCAAATGAAGAGAAAAGTAGGACTTACAGCCATGGGACTAGAATCT90410.10229090442537991No Hit
CTTACCAGCCTGGTGGATCCAGAAAGGGGAGCCCAGGTAGCTGTGAACAG88920.10060510144347729No Hit
CAGCTGTATGACCGTGGGACCAGTGGAGTTCTTGGGATCTCTGGGGCCAT88700.10035619093608228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)