FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003472174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8838518
Sequences flagged as poor quality0
Sequence length8-343
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCAAGGCTTCAGCTCTGACAGCAGAGGGTGGAAGCTGCCTCATCTC130420.1475586744293557No Hit
CTCTCAACAGGAACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTG117650.13311055088647214No Hit
GATGTTATCCGTGCGCAGGATCTGCTTCTTGAGGTGGCTCATGAGGTCTC103200.11676165619620846No Hit
CTAGGACCAACCAGATTGGACCATGACAGCCATTCTGTATGTTGTGGACG100630.11385392890527575No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT96360.1090228022390179No Hit
GTCTTGCCCTCAGGCCTGCCGGCTCCCCCCAGTATCATCAAGGCCATGGG93910.10625084431575518No Hit
CTTCCAGAATGCATTGAACGAATCCCATGGATTGCTCCTGAGTGTGTTGA93080.10531177285603763No Hit
GGATTCAAATGAAGAGAAAAGTAGGACTTACAGCCATGGGACTAGAATCT90350.10222301974154491No Hit
TCCTTAGATGTCTCCTTGCTGGCATCAGACTCAGAGCCCCTGAAGTGTTT90340.1022117056275724No Hit
CTTACCAGCCTGGTGGATCCAGAAAGGGGAGCCCAGGTAGCTGTGAACAG88740.10040144739197228No Hit
CAGCTGTATGACCGTGGGACCAGTGGAGTTCTTGGGATCTCTGGGGCCAT88450.10007333808676976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)