FastQCFastQC Report
Wed 8 May 2024
EGAF00003472175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3400368
Total Bases465.7 Mbp
Sequences flagged as poor quality0
Sequence length8-360
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG49810.14648414524545578No Hit
CTGACCCTTCCCGCTGCTGTCTAGAACTGCTTTCTGGAGTGTGCGTACCA44280.13022119958780934No Hit
CTTACCAGCCTGGTGGATCCAGAAAGGGGAGCCCAGGTAGCTGTGAACAG40020.11769314380090626No Hit
CTGCCGACCAACGAACTGGTCCCTTTGTTCTTCCCTCTCCACAGGCAAAG39060.11486991996160416No Hit
CAGAGGAGTTGGTGGGTGTGAGTGCCCCTGTCCCTGCACTTCGGGTGGCT38090.11201728754064266No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC37300.10969400958955032No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT36070.10607675404544449No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC34340.10098906941836884No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC34150.1004303063668403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)