FastQCFastQC Report
Wed 8 May 2024
EGAF00003472181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728635
Total Bases380.4 Mbp
Sequences flagged as poor quality0
Sequence length8-307
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG44110.161655919534859No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC38630.14157261781073688No Hit
CAGAGGAGTTGGTGGGTGTGAGTGCCCCTGTCCCTGCACTTCGGGTGGCT36840.1350125612256678No Hit
CTGACCCTTCCCGCTGCTGTCTAGAACTGCTTTCTGGAGTGTGCGTACCA35730.13094459317570872No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC34270.12559393249738424No Hit
CCAGTTGCCGGATCTCCAAGTACTCAAAGTCCATCTCCTCCGCCAGCGCG34130.12508085544603803No Hit
ACCGAAGATTGCAGCCTTCTTGCCTGGGGACCATGCCCCTGTCTGTGTGC32750.12002338165419706No Hit
GATGTTATCCGTGCGCAGGATCTGCTTCTTGAGGTGGCTCATGAGGTCTC32540.11925376607717778No Hit
CAGCAAGCGCAAGCGGGACGAGTGGCTGGCACGCTGGAAAAGGGAGGTGA31250.11452612753263078No Hit
CCACATCCTCCCTTCCCCTGGCTGCTCTCAACATGCGAGTGCGGCGCCGC30440.11155761030698501No Hit
AGGATCCCATATTTTTGAGCCTGGGGTTGCTCCAGGAGTCTCGCTTCCCT30260.11089793981239704No Hit
CTTACCAGCCTGGTGGATCCAGAAAGGGGAGCCCAGGTAGCTGTGAACAG30100.11031156603942997No Hit
CCCATGGGAACAGTATTGGACCCTTACCCAGGGGGTCATCAAGTGTGGTG29930.10968854390565246No Hit
TCCATTTCAGTGCACCATAAGATGTCCTCTTTCTCATTCATGAAGACAGG29350.10756293897864683No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC29150.106829971762438No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG28530.1045577733921906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)