FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003472181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003472181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728635
Sequences flagged as poor quality0
Sequence length8-307
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG43620.15986014985514735No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC38280.1402899251823714No Hit
CAGAGGAGTTGGTGGGTGTGAGTGCCCCTGTCCCTGCACTTCGGGTGGCT36530.1338764620405441No Hit
ACCGAAGATTGCAGCCTTCTTGCCTGGGGACCATGCCCCTGTCTGTGTGC32420.11881398574745247No Hit
GATGTTATCCGTGCGCAGGATCTGCTTCTTGAGGTGGCTCATGAGGTCTC32200.11800772180962275No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC31270.11459942425425167No Hit
CAGCAAGCGCAAGCGGGACGAGTGGCTGGCACGCTGGAAAAGGGAGGTGA30960.11346332506912797No Hit
AGGATCCCATATTTTTGAGCCTGGGGTTGCTCCAGGAGTCTCGCTTCCCT30110.11034821440024042No Hit
CTTACCAGCCTGGTGGATCCAGAAAGGGGAGCCCAGGTAGCTGTGAACAG29830.10932206029754803No Hit
TCCATTTCAGTGCACCATAAGATGTCCTCTTTCTCATTCATGAAGACAGG28450.10426458650570707No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG27810.10191909141383879No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC27380.10034321189898979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)