FastQCFastQC Report
Mon 6 Feb 2023
EGAF00003474398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123146413
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT5645010.4583982482705363TruSeq Adapter, Index 19 (97% over 40bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG2362590.19185211671573416Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA1693990.13755902090302866TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)