FastQCFastQC Report
Tue 14 Feb 2023
EGAF00003474714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118844729
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2536980.213470132108257No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2449560.2061143157640588No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2101190.1768012782460045No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1611560.13560214353301273No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1533750.12905494529757394No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1412110.11881974168160205No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1361810.11458732847966695No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATAGTCGGGTGTAGATCT1353760.11390997408055009Illumina Single End PCR Primer 1 (97% over 35bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1241930.10450021725406097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)