FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003474741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406188516
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG9046450.22271555309062455TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1908200.081.105891
TCGGAAG2077800.074.4254762
CGGAAGA2278300.067.834973
AGAGCAC4721750.033.035137
AGCACAC5037700.030.9891859
GAGCACA5151050.030.352228
GAAGAGC5163950.030.3519215
CGCGCAT1411000.022.33767735-39
TCGCGCA1462300.021.58179335-39
TCTCGCG1495750.021.4797530-34
AAGAGCA7445950.021.3254096
TATGCCG1555900.020.71769545-49
GCGCATC1582450.020.06317935-39
CTCGTAT1510650.019.593340-44
TCTCGTA1537600.019.1122240-44
CGTATGC1673450.019.05620845-49
GGAAGAG8513400.018.8327664
CTCGCGC1662900.018.56042930-34
ATGCCGT1812200.017.76438345-49
CGCATCT1656000.017.74330935-39
Produced by FastQC (version 0.11.5)