FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003474761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342828574
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG3538580.10321718399120372TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1300600.047.7929951
TCGGAAG1382600.044.749532
CGGAAGA1612450.038.417773
AGAGCAC3498000.018.0708527
AGCACAC3731600.017.019289
GAGCACA3804750.016.6653798
GAAGAGC3990800.016.211565
TATGCCG909800.014.69714945-49
CTCGTAT891400.013.78207840-44
TCTCGTA910200.013.51811840-44
CGTATGC984050.013.43350245-49
TATCTCG949950.012.859364540-44
CGCTCAT1084750.012.36557830-34
ACCGCTC1108950.012.1401830-34
ATGCCGT1135900.011.83678745-49
GCCGTCT1143300.011.64231150-54
ATCTCGT1091650.011.43320840-44
GTCACCG1213300.011.24312125-29
AAGAGCA5867950.011.2293726
TCGTATG1042600.011.02970340-44
Produced by FastQC (version 0.11.5)