FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003474775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411845398
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG8673370.21059771560200852TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2175300.068.371851
TCGGAAG2337900.063.5834852
CGGAAGA2563000.057.937763
AGAGCAC4983850.030.1457927
AGCACAC5291500.028.4092089
GAGCACA5434150.027.7542978
GAAGAGC5482250.027.5332895
ACCGCGC1176050.020.7702541
AAGAGCA7797050.019.6510776
TATGCCG1651250.018.7663645-49
CTCGTAT1597250.018.0993540-44
TCTCGTA1635400.017.65672540-44
GGAAGAG8883550.017.3349844
CGTATGC1770800.017.31268545-49
TATCTCG1689200.016.88817440-44
CGCTCAT1893850.016.39239930-34
ATGCCGT1899050.016.35426145-49
TCGTATG1752800.015.89369740-44
GCCGTCT1938000.015.80944850-54
TGCCGTC1887300.015.80370845-49
Produced by FastQC (version 0.11.5)