FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003474783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430668837
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG10821190.25126475542970383TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2275600.081.723711
TCGGAAG2451400.075.741092
CGGAAGA2695250.068.831913
AGAGCAC5166800.036.0523387
AGCACAC5502850.033.8885279
GAGCACA5638050.033.0736588
GAAGAGC5709300.032.885985
AAGAGCA8069850.023.5247296
TATGCCG1804350.020.97874345-49
GGAAGAG9257650.020.6864034
CTCGTAT1757800.020.11723140-44
TCTCGTA1786100.019.76033240-44
CGTATGC1922300.019.49295245-49
TTACTCG2001650.018.58012430-34
ATGCCGT2053300.018.4613545-49
TACTCGA2063400.018.15559235-39
TCGTATG1941650.017.9107140-44
TGCCGTC2086900.017.83378245-49
GCCGTCT2116850.017.67553950-54
ATCTCGT2016700.017.51017840-44
Produced by FastQC (version 0.11.5)