FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003474791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410828586
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG14984830.3647465271562189TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2787400.094.82081
TCGGAAG2981650.088.394942
CGGAAGA3191150.082.4853
AGAGCAC5641550.046.8220757
AGCACAC6003650.044.0047659
GAGCACA6079550.043.432118
GAAGAGC6145200.043.1737635
AAGAGCA8437150.031.6523636
GGAAGAG9548650.028.2719254
TATGCCG2367400.022.7000145-49
CTCGTAT2235900.022.0026740-44
TCTCGTA2260000.021.69618840-44
CGTATGC2478500.021.42365645-49
ATGCCGT2616200.020.53902445-49
TCGTATG2367300.020.40839840-44
TGCCGTC2611900.020.1322145-49
GCCGTCT2654200.019.8634750-54
ATCTCGT2489150.019.75360140-44
CGTCTTC2898000.018.57372950-54
CGTCTGA2893650.018.54733315-19
Produced by FastQC (version 0.11.5)