FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003474795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412958375
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG15264540.3696387075331745TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2847850.091.920951
TCGGAAG3045150.085.599252
CGGAAGA3249000.080.053593
AGAGCAC5668050.046.125167
AGCACAC5995800.043.605729
GAGCACA6113550.042.750938
GAAGAGC6202450.042.380495
AAGAGCA8424800.031.3307066
GGAAGAG9582150.027.7898734
TATGCCG2430300.022.95501545-49
CTCGTAT2322700.022.35877640-44
TCTCGTA2360500.021.95405440-44
CGTATGC2562850.021.4937145-49
TATCTCG2414650.021.15128940-44
ATGCCGT2684650.020.78398945-49
ATCTCGT2577450.020.1488840-44
GCCGTCT2731900.020.065650-54
TCGTATG2471650.019.65925240-44
TGCCGTC2706200.019.30673245-49
CGTCTTC2959050.018.91391250-54
Produced by FastQC (version 0.11.5)