FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003474801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398138945
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG16901970.424524408181169TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3160900.092.765131
TCGGAAG3326450.087.8797762
CGGAAGA3527250.082.6989443
AGAGCAC5889250.049.800647
AGCACAC6186000.047.3148549
GAGCACA6293350.046.5669758
GAAGAGC6373600.046.0887265
AAGAGCA8596400.034.3670966
GGAAGAG9661400.030.8069324
TATGCCG2661100.022.56300445-49
CTCGTAT2513350.022.2426440-44
TCTCGTA2540850.021.950540-44
CGTATGC2771500.021.3460945-49
TATCTCG2594800.021.10764340-44
ATGCCGT2896200.020.69689445-49
TCGTATG2664400.020.40375340-44
ATCTCGT2754950.020.24409740-44
TGCCGTC2893200.020.16097345-49
GCCGTCT2926200.020.12457750-54
CGTCTTC3129050.019.18033250-54
Produced by FastQC (version 0.11.5)