FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003474812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348542142
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1817450.020.5127269
GAGCGTC1978850.018.9276228
ATCGGAA2075250.018.5602631
TCGGAAG2229950.017.2323172
AGAGCGT2196250.017.0803097
CGGAAGA2502000.015.3711583
AAGAGCG2667400.014.26176556
GAAGAGC4814050.08.4080825
CGCCGTA1147700.07.066859255-59
GCCGTAT1177150.06.947928455-59
CGTATCA1242100.06.56134660-64
CCGTATC1232200.06.352871455-59
ACCGCGC1490850.05.8415511
TCGCCGT1524250.05.52074855-59
GGAAGAG7797150.05.490764
TGGTCGC1701100.05.063521450-54
TCGTGTA1621300.05.033121610-14
Produced by FastQC (version 0.11.5)