FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003474817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342326908
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG5273930.154061216829616TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1439300.065.310551
TCGGAAG1555900.060.417572
CGGAAGA1724800.054.4276163
AGAGCAC3720700.025.6283237
AGCACAC3969700.024.009849
GAGCACA4058000.023.5927548
GAAGAGC4118800.023.2706955
TATGCCG1125100.018.69861845-49
CTCGTAT1070950.017.96792840-44
TAATGCG1178500.017.84005730-34
TCTCGTA1099700.017.3821740-44
CGTATGC1202500.017.2563545-49
GCGCATC1237550.016.85426735-39
GATCGGA654100.016.8133491
AAGAGCA6062700.016.2075256
TGCGCAT1299300.016.0398735-39
CGCATCT1223050.015.661262535-39
ATGCCGT1363050.015.48542245-49
ATGCGCA1353700.015.39635935-39
AATGCGC1243550.015.21056630-34
Produced by FastQC (version 0.11.5)