FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003474819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003474819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367404934
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG3805900.10358870139724362TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1319100.049.9411131
TCGGAAG1450250.045.4007762
CGGAAGA1671250.039.3326343
GATCGGA728750.021.6673851
AGAGCAC3753350.018.0943497
AGCACAC4022200.016.7839839
GAGCACA4100050.016.5289428
GAAGAGC4207650.016.1990435
TATGCCG1020100.016.05555745-49
CTCGTAT964000.015.63312440-44
CGTATGC1077150.015.10155545-49
TCTCGTA1001200.015.03199740-44
CGGCTAT1097400.014.95225630-34
ATGCCGT1316000.012.62506845-49
ATCTCGT1230650.012.43078340-44
ACCGGCT1360750.012.25027830-34
GCCGTCT1342450.012.1843650-54
ACACGTC1388400.011.95908610-14
CGTCTGA1388200.011.94840415-19
GTCACCG1463200.011.46506225-29
Produced by FastQC (version 0.11.5)