FastQCFastQC Report
Wed 14 Sep 2022
EGAF00003477197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003477197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59276135
Sequences flagged as poor quality0
Sequence length35-51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT9225271.5563211062934519No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2042050.3444978320533213No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1391350.23472346839077818No Hit
ACCGCATCTCCCACAGGGAATTCGAAGGACCCTCGAGCCACCACCTTC1348900.22756207030029876No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1179530.19898901978005146No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1065330.1797232562480668No Hit
CGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGA922390.1556089984611851No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC915230.15440109244639516No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT896250.1511991292954576No Hit
CCGCATCTCCCACAGGGAATTCGAAGGACCCTCGAGCCACCACCTTCAG881640.1487343936982396No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG880620.14856231770171924No Hit
CACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCCC879910.1484425393119845No Hit
CGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGG826920.13950302259079475No Hit
CCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCCG797380.13451956676999943No Hit
CTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCG778180.13128048918843982No Hit
CCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCCGG745950.1258432251023114No Hit
GCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCC684020.11539551288220799No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA671970.11336265429586459No Hit
GGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTC600440.10129540328498138No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTT595970.10054130553552455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)