FastQCFastQC Report
Wed 14 Sep 2022
EGAF00003477265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003477265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60344177
Sequences flagged as poor quality0
Sequence length35-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT6734201.1159651742371097No Hit
GTTCAGAGTCTTCCTGTCGTATTGGGGGTGGCGGATGATCTTGGCTGCATT1228980.20366173856344086No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1017830.1686707898924531No Hit
CCAGAAATGACATCACAGTATGTGCCAGCAGGAAGACCAGTTTGCAAAGTT858120.1422042759817571No Hit
GGGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAGT756800.12541392353399733No Hit
GTCGTATTGGGGGTGGCGGATGATCTTGGCTGCATTGATGAACTGCTCATT742750.12308561271786007No Hit
CTCCATTCCACTTGCGAATAACTGTGCCGAGTTTTGCACCATACTTGAATT693250.11488266713787479No Hit
CCAGAAGGTAAGAATAGAGGCTCCTCCAGCCCCATGTCCTCGTTGATTGTC690400.11441037633175442No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG671880.11134131467233367No Hit
CTCGTTGATTGTCATGGTTATCCACAAAGACAAGCGCTCTGTCAGAAGGT657530.10896328903449955No Hit
GTCATTGTTCAGAGTCTTCCTGTCGTATTGGGGGTGGCGGATGATCTTGGC611260.10129560636811734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)