FastQCFastQC Report
Sat 1 Apr 2023
EGAF00003477310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003477310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40209746
Sequences flagged as poor quality0
Sequence length35-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6954951.7296677278190218No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1844550.45873206958332935No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA1410770.3508527509723638No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTGG758500.18863585957493986No Hit
GTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGA641210.15946631446018086No Hit
CTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGG619520.15407209983370698No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGA597320.14855105028517215No Hit
AGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAA536080.13332091180083555No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGAC464010.11539739644214615No Hit
AAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGA457000.11365403800362231No Hit
GGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGA455480.1132760201966956No Hit
GGAAAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGG440030.10943366814602608No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGG425390.10579275979510042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)