FastQCFastQC Report
Tue 27 Sep 2022
EGAF00003477323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003477323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62026185
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT7200541.160887131781521No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3929190.633472782503067No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG2094270.33764288421091837No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1740450.28059923401705267No Hit
CCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACA1438540.2319246298962285No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1418620.22871308303098117No Hit
GCCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACAC1118340.1803012711486286No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1007370.16241044004237887No Hit
CCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACAC907600.14632529793666982No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC881770.14216092767917293No Hit
GTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGC631270.10177475851529479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)