FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003478545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003478545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10627596
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT278150.2617242883526999TruSeq Adapter, Index 6 (97% over 36bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG120520.11340288057619051No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT111520.1049343614491932No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG110710.10417219472776346TruSeq Adapter, Index 6 (97% over 35bp)
GCCGTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGG108440.1020362460146208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)