FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003478610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003478610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6268197
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT160150.2554961179426875Illumina Single End PCR Primer 1 (96% over 33bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC150230.2396701954325941Illumina Single End PCR Primer 1 (96% over 32bp)
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC99970.15948764852157646No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN94920.15143110530827286No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA93550.14924546883258455No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG83590.1333557321188214No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC62910.10036378882157024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)