FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003478706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003478706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12061585
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT298780.24771205442734104Illumina Single End PCR Primer 1 (96% over 33bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN196000.16249937300943448No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC194350.16113139359379386No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA176140.14603387531572343No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG159050.1318649248834212No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC121050.10035994440200025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)