FastQCFastQC Report
Fri 30 Dec 2022
EGAF00003490644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351365036
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA167175964.757899701779092TruSeq Adapter, Index 1 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA115470743.2863469090305277TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC6035890.17178402463471065TruSeq Adapter, Index 1 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC4199340.1195150219784532TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3787110.10778277893307517TruSeq Adapter, Index 1 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)