FastQCFastQC Report
Mon 23 Jan 2023
EGAF00003490648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346511268
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA314903569.087830298205484TruSeq Adapter, Index 2 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA88648982.55832892568446TruSeq Adapter, Index 2 (100% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6159000.17774313763441596TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6101100.17607219630156443TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5895340.1701341498655103TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5823270.16805427522201097TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5685850.16408845902234845TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5638860.16273237036551436TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5523080.1593910648816188TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5334060.15393611961848236TruSeq Adapter, Index 2 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5295120.15281234663918633TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5163480.14901333598190522TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4591640.13251055374049192TruSeq Adapter, Index 2 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)