FastQCFastQC Report
Mon 26 Dec 2022
EGAF00003490651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338316262
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAAA14396750.4255411760254078Illumina Single End PCR Primer 1 (100% over 56bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA14176580.4190333599748746Illumina Single End PCR Primer 1 (100% over 57bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGAA5209740.15399023296137032Illumina Single End PCR Primer 1 (100% over 56bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGA3821870.11296737488782019Illumina Single End PCR Primer 1 (100% over 57bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)