FastQCFastQC Report
Mon 23 Jan 2023
EGAF00003490654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317081299
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA235147327.415994596388985TruSeq Adapter, Index 7 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA59455381.875083147051192TruSeq Adapter, Index 7 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC7200390.22708340172404806TruSeq Adapter, Index 7 (100% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4767680.15036143774597063TruSeq Adapter, Index 7 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4477060.14119596501337658TruSeq Adapter, Index 7 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4363710.1376211720389098TruSeq Adapter, Index 7 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4151880.13094055099099364TruSeq Adapter, Index 7 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4151770.13093708184915692TruSeq Adapter, Index 7 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4144850.13071884128997466TruSeq Adapter, Index 7 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4121740.12999000612773445TruSeq Adapter, Index 7 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4042830.12750136992468925TruSeq Adapter, Index 7 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4028150.12703839717775348TruSeq Adapter, Index 7 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3859210.12171042606962451TruSeq Adapter, Index 7 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3722930.11741247471046849TruSeq Adapter, Index 7 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA3548360.11190694661560598TruSeq Adapter, Index 7 (100% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3467450.10935523510643874TruSeq Adapter, Index 7 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)