FastQCFastQC Report
Fri 30 Dec 2022
EGAF00003490655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317081299
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAAA15676810.49440979488355136Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGAA7509460.23683074415561797Illumina Single End PCR Primer 1 (100% over 56bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA5176610.16325813021221414Illumina Single End PCR Primer 1 (100% over 57bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAAGAA4026450.12698478316754974Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAAAAA3214190.10136800909220445Illumina Single End PCR Primer 1 (100% over 56bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)