FastQCFastQC Report
Wed 15 Feb 2023
EGAF00003490658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258722337
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA67140602.5950832378265045TruSeq Adapter, Index 14 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA19098440.738182880591404TruSeq Adapter, Index 14 (97% over 44bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC9833060.3800622750249817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)