FastQCFastQC Report
Mon 26 Dec 2022
EGAF00003490674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374020080
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA294672437.878518982189406TruSeq Adapter, Index 4 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC22550740.6029285914274977TruSeq Adapter, Index 4 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA12769830.3414209739755149TruSeq Adapter, Index 4 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6620290.17700359830948115TruSeq Adapter, Index 4 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6008310.16064137519033736TruSeq Adapter, Index 4 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4680920.12515156940237007TruSeq Adapter, Index 4 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAA4630150.12379415565068057TruSeq Adapter, Index 4 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAG4027650.10768539485901399TruSeq Adapter, Index 4 (100% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)