FastQCFastQC Report
Wed 8 Feb 2023
EGAF00003490676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371839761
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA285170277.669170968512967TruSeq Adapter, Index 4 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC25657910.6900259921369732TruSeq Adapter, Index 4 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA13316030.35811205246552424TruSeq Adapter, Index 4 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6489290.1745184533936918TruSeq Adapter, Index 4 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6147100.16531583345117307TruSeq Adapter, Index 4 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5452410.14663332359446088TruSeq Adapter, Index 4 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAA4450940.11970048571540472TruSeq Adapter, Index 4 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAG4315070.11604649240294666TruSeq Adapter, Index 4 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAT3744880.10071219898401344TruSeq Adapter, Index 4 (100% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)