FastQCFastQC Report
Mon 19 Dec 2022
EGAF00003490680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375198863
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA309544518.25014520366497TruSeq Adapter, Index 10 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC30203310.8049947102318378TruSeq Adapter, Index 10 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA17996420.4796501742064181TruSeq Adapter, Index 10 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6725670.17925614022982794TruSeq Adapter, Index 10 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6656070.17740112394743585TruSeq Adapter, Index 10 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAT6151360.16394932412148594TruSeq Adapter, Index 10 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5659750.1508466724751242TruSeq Adapter, Index 10 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAG5184020.13816726304951515TruSeq Adapter, Index 10 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3995050.10647820113463403TruSeq Adapter, Index 10 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATA3877160.10333613404366847TruSeq Adapter, Index 10 (100% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)