FastQCFastQC Report
Fri 30 Dec 2022
EGAF00003490682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379925864
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA283453547.4607592390709145TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC18645280.49076100804761214TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA9387620.24709083770090473TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6013460.1582798269296033TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAT3998810.10525237628991745TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3887200.10231469790116737TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAG3804940.10014953864788737TruSeq Adapter, Index 12 (100% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)