FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003490700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914544
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA226191.1814301473353446No Hit
CACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATAT166620.8702855614705121No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT139780.7300955214400923No Hit
GGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACC103730.5418000317569092No Hit
ACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATA76110.3975359145572No Hit
ACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTA72160.3769043699178499No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC66240.345983168838115No Hit
ATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGA56640.295840680600707No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC54860.28654342757335427No Hit
GCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATT54550.28492424305735464No Hit
TCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGA49630.25922621783568306No Hit
GGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAG45990.24021385771233256No Hit
GGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTC43200.22564119706833585No Hit
AGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTA37270.1946677642300203No Hit
CGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACCAGAGTTT37130.19393651960989144No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG33720.17612548993389548No Hit
GGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTA32310.1687608119740262No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG30870.161239438738415No Hit
ACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAG30010.15674750750048053No Hit
CTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCC30010.15674750750048053No Hit
CCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCC29890.15612072639751293No Hit
GGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGG29310.1530912843998362No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG28460.1486515849204824No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG27390.14306278675235462No Hit
GTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAA27110.14160029751209688No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG26220.1369516709984205No Hit
GCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTC25560.1335043749320987No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA25530.1333476796563568No Hit
CACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTT25300.13214634920900226No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG24720.12911690721132552No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCC24530.1281245037982935No Hit
CGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGG24180.12629639224797132No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT24140.12608746521364878No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT23740.12399819487042346No Hit
CCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGC23670.12363257256035902No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA23530.12290132794023016No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT23130.12081205759700482No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA22920.11971519066681152No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC22260.11626789460048974No Hit
GACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCC22210.11600673580758655No Hit
ACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCC22040.1151187959117158No Hit
GACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACT21950.11464871008449008No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG21930.11454424656732884No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA21440.1119848903968778No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA21200.11073132819094259No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC20950.10942553422642676No Hit
TAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT20670.10796304498616903No Hit
GTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGG20560.10738849564178206No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG20550.10733626388320142No Hit
GAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT20430.10670948278023384No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG20280.10592600640152432No Hit
CCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGA20250.10576931112578243No Hit
TCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGA20140.10519476178139546No Hit
CTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTAT19740.10310549143817015No Hit
CGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTA19450.10159077043933176No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA19440.10153853868075113No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC19260.10059836702629975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)