FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003490701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914544
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGA309431.616207305760536No Hit
GTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAG226811.1846685163673438No Hit
CCGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGG208631.0897111792677525No Hit
CGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGT204401.0676171453881447No Hit
CCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGG176860.9237708822570806No Hit
GTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAG163090.8518477506915485No Hit
AGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGAT144280.7535998128013772No Hit
GAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAAT130680.6825646211317159No Hit
AACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTT126500.6607317460450113No Hit
CCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGTAG124000.6476738063998528No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG109550.5721989152508378No Hit
CGCCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGT103590.5410687871367803No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC96370.5033574574415631No Hit
GAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTG72530.3788369449853333No Hit
GGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGA71200.3718901210941091No Hit
TGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAG69900.3650999924786268No Hit
CAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAA68780.3592500355175958No Hit
GGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTT66970.34979608721450123No Hit
GAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAG65850.34394613025347026No Hit
GGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCG63090.32953016488521547No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA57990.3028919680090925No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT53780.2809023976466459No Hit
AAGGTGTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAAT53460.2792309813720656No Hit
GAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGA48120.25133922229000744No Hit
CGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAG46470.24272098212420293No Hit
CGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTG44740.23368488788975336No Hit
CGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGA44150.23060321413349602No Hit
GAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAG42910.2241264760694975No Hit
GGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCG41960.21916445900433731No Hit
GAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATG39040.2039127854987924No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC35120.18343793613518414No Hit
GAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGC31350.1637465631502854No Hit
GCCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGTA30990.1618662198413826No Hit
GATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGC30380.15868008256796395No Hit
AAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTG28570.14922613426486933No Hit
GGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTC28400.14833819436899856No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCG26810.14003334475467788No Hit
GCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGA26020.13590703582680783No Hit
AGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGC25850.13501909593093708No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC25230.1317807268989378No Hit
CACGCCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGA25210.13167626338177654No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC24790.12948252952138994No Hit
CGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACC22080.11532772294603832No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT21580.11271613501700666No Hit
GGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGG20580.10749295915894333No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA20130.10514253002281483No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC20000.1044635171612666No Hit
GGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAA19180.10018051295765466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)