FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003490702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823634
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATAT186801.0243283465870894No Hit
CCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTC174910.9591288602866584No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT137770.7554695733902747No Hit
CACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAT109830.6022590059189509No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC95560.5240086552455152No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC78980.43309128915122225No Hit
CGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACTTCG76070.4171341398548174No Hit
GGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACC74960.4110473921850547No Hit
ACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATA71230.39059372659206837No Hit
CCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGT68530.37578812415210505No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA62170.34091270507130267No Hit
ACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTA60500.3317551657843624No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC59820.3280263473920754No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA52950.29035431451705773No Hit
GCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATT48080.2636493945605313No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA44740.2453343159866508No Hit
CGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGC44030.24144099090058643No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA40760.2235097612788531No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA39230.21511991989620724No Hit
AGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATT39200.21495541320242986No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG39150.2146812353794676No Hit
GCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATC37830.2074429408532633No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG35840.19653066349936443No Hit
ACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGC34540.1894020401023451No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC33210.18210891001154839No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA31940.1751447933083064No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC30210.1656582406338114No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA30120.16516472055247927No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG30070.164890542729517No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC29850.16368416030848296No Hit
GCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCG28370.15556849674879936No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG27730.15205902061488216No Hit
CTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCC27590.15129132271058776No Hit
CCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTC26280.14410786374897594No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA25520.13994036083994923No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG25390.1392274985002473No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT24980.1369792403519566No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC24560.1346761466390734No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC23310.12782170106501634No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT22650.1242025538019142No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC22630.12409288267272928No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG22460.12316067807465751No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG22420.12294133581628769No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG22330.12244781573495558No Hit
CTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTAT21940.12030922871584977No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT21530.11806097056755907No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG20800.11405797435230973No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA20550.11268708523749832No Hit
GGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCA20440.11208389402698128No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT20210.11082267604135478No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCC20130.11038399152461513No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC19360.10616165305099598No Hit
GACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACT19260.10561329740507142No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA19100.10473592837159211No Hit
GGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGG19000.10418757272566755No Hit
CCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCC18600.10199415014196928No Hit
GTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGG18530.10161030118982209No Hit
CTGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTT18470.10128128780226733No Hit
CACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCC18360.10067809659175032No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)