FastQCFastQC Report
Fri 31 May 2024
EGAF00003490703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823634
Total Bases184.1 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAAC221771.21608831596691No Hit
CGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGT186231.0212027194053193No Hit
CCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGTAG174170.9550710285068166No Hit
AGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGAT158490.8690888632258446No Hit
CCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGG154040.8446870369822015No Hit
CGCCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGT151860.8327328839010458No Hit
CCGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGG141480.7758135678540761No Hit
GCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCC138320.7584855294428596No Hit
CCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAA107260.5881662658186895No Hit
GGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGG96780.5306985941257949No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC87970.48238846171984073No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG84080.4610574270933751No Hit
GAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCC76740.42080812268251194No Hit
GGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTG74450.4082507783908394No Hit
CGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC69670.3820393785156451No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGA68910.37787187560661845No Hit
CAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAA68590.3761171375396598No Hit
GGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCG65990.3618598907456211No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT65530.3593374547743681No Hit
GGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTT55030.3017601119522887No Hit
GAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTG54490.298798991464296No Hit
TAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCT54430.2984699780767413No Hit
CGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAG51350.28158062418226465No Hit
CGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGA50610.27752279240242284No Hit
GAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAG45620.25015984567078703No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT38580.2115556081976976No Hit
TGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAG37750.20700425633652367No Hit
CGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTG37590.20612688730304438No Hit
AGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTT37340.20475599818823292No Hit
GGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCG36950.20261741116912713No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA36320.19916277059980236No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCG35280.19345987188218688No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA34670.19011490244204704No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT34580.1896213823607149No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC33960.18622157735598263No Hit
GATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGC30250.1658775828921812No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC28940.15869412393056942No Hit
CACGCCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGA28690.157323234815758No Hit
CGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACC28650.15710389255738816No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG26620.14597227294511947No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC25990.1425176323757947No Hit
GGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTC24680.13533417341418288No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT24500.13434713325151867No Hit
GAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGAG24310.133305257524262No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT24060.13193436840945058No Hit
TGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCC22560.12370903372058209No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG22510.12343485589761981No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA22330.12244781573495558No Hit
GAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAG22240.12195429565362349No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT21980.12052857097421962No Hit
GCCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGTA21910.12014472202207241No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC20670.11334511201260779No Hit
GGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGA20620.1130709341896455No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCC20520.11252257854372096No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC19950.1093969513619509No Hit
GAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACC18600.10199415014196928No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA18410.10095227441471259No Hit
CCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGC18310.10040391876878804No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)