FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003490704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733801
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGA260770.9538733799570634No Hit
CACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATAT168310.615662954253071No Hit
CACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAG152560.5580508603223132No Hit
GCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATG128520.4701146864749848No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC116890.427573184734368No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT116620.4265855488384122No Hit
CACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAT96430.3527323312852691No Hit
ATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTG82920.30331395738021893No Hit
GGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACC80910.29596155682143654No Hit
TACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCA77870.28484150821511883No Hit
ACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATA71680.2621990408226495No Hit
ACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTA69770.2552124313364433No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG55610.20341641545964756No Hit
CGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGC53740.19657612240247188No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA42220.1544369908416889No Hit
GCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATT41780.15282751012235346No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG41480.15173013690462472No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA37570.13742770596689372No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT37240.13622059542739212No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA36760.13446479827902616No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT34570.1264539737896065No Hit
TCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGG33570.12279606306384407No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG32810.12001605091226464No Hit
CGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACTTCG31920.1167605103663361No Hit
CTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTAT30510.1116028562430111No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG30080.11002995463093328No Hit
AGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATT29880.1092983724857808No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT28720.10505519604389639No Hit
GCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAG28560.10446993032777441No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC28390.1038480855043948No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG28200.10315308246649994No Hit
CAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGG28070.10267755407215083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)