FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003490705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733801
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT240000.8778985741829781No Hit
CGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAAC203340.7437995669765283No Hit
TTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATT180610.6606552561799487No Hit
CCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAA143310.5242151861090109No Hit
AGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGAT134360.4914768851134373No Hit
GGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACT118830.43466953154234705No Hit
GAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAAT107410.39289619105414036No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTA103320.37793533618577213No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG97120.35525628968604517No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA94340.3450872978684257No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC91380.33425988212016894No Hit
GAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAG73530.26896617566530995No Hit
CAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAA68890.2519934698977723No Hit
GAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTG65470.23948341521566494No Hit
TAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCT56950.20831801583216922No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT53930.19727112544036673No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT53520.19577138204280414No Hit
GATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC52670.1926621579259061No Hit
GTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGC50340.1841392259348797No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATT50240.18377343486230344No Hit
AGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTT47950.1753968193003075No Hit
GAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGA45760.16738599481088784No Hit
CGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTG44630.16325255569077632No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC44020.16102123014806125No Hit
CGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAG43420.1588264837126038No Hit
GAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATG38090.13932981954429016No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC38070.13925666132977493No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA36940.1351232222096634No Hit
GGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC36060.1319042607709925No Hit
CGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG34660.12678318575492512No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC32710.1196502598396884No Hit
TGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCA32640.11939420608888503No Hit
GAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTG32580.1191747314453393No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG32030.11716288054616995No Hit
GATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGC31560.11544366250506163No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC30800.1126636503534822No Hit
GGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGA30720.11237101749542121No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT29710.10867652766240118No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCG28670.10487230050760826No Hit
CGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCT28440.10403098104068292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)