FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003490706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5658792
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTT338730.5985906532701679No Hit
CCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGA319260.564184016659386No Hit
CCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTC313760.5544646277862837No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC266330.4706481524678765No Hit
GACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACT243020.4294556152620559No Hit
CACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAG205940.3639292626412139No Hit
CTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTA187710.33171390643091314No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC150340.26567507694221665No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC136430.24109385890133442No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT128020.22623202973355447No Hit
ATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTG121780.2152049412666166No Hit
GCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATG121770.21518726965048368No Hit
CACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAT115930.20486704582886242No Hit
GGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAA109670.19380461412965877No Hit
CCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGT104630.18489811959867053No Hit
GTCGGTGACAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT103420.18275985404658804No Hit
GGCCTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG91350.16143021337416186No Hit
CTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCT89970.15899153034781982No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC86840.153460314498218No Hit
GGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCAC84660.14960790218124292No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG82130.14513698329961588No Hit
GGCGTGGTGTCACCTGTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGG77630.13718475603980496No Hit
GGCGTGTCCTGTGGATGCTGAGGAAGTGTCGGTGACAAGAAGAGGGGTGG75670.13372111927775399No Hit
GTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGT74070.13089366069648786No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA73510.12990405019304474No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGTGGATGCTGAGGAAGGGC71760.12681151736978494No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCTGAGGAAGGGC71450.12626369726966463No Hit
GGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACC70580.12472626666610118No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT69720.12320650767867064No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA69560.12292376182054404No Hit
GTCGGTGACAAGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGGGC68520.12108591374272105No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA68260.12062645172326533No Hit
GCCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGT67770.11976054253275259No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT65750.11619087607390412No Hit
CTGAGGAAAGGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCT64860.11461810223807484No Hit
GCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTGT61290.10830933527862484No Hit
GGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGG59460.10507542952630172No Hit
ACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTA59170.10456295265844724No Hit
CGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACTTCG58460.1033082679130104No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG57320.10129370367385832No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC57160.10101095781573169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)