FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003490707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5658792
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1799863.1806435012985106No Hit
TGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGA345890.6112435304213337No Hit
CGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC300860.5316682429748257No Hit
GTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAG239560.423341236080068No Hit
CGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAAC234070.41363951882309863No Hit
GTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAG203250.35917559790146025No Hit
TTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATT202740.35827434547868164No Hit
CCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGTAG184400.3258646014909189No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGA174150.3077511949546829No Hit
CGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGT171220.30257341142773936No Hit
CGCCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGT152770.26996927966251455No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC140400.24810949050610093No Hit
CCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGG140270.24787975949637306No Hit
AGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGAT139280.24613026949921465No Hit
CCGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGG128630.22730999831766216No Hit
CGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG122130.21582344783126856No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA110270.19486491109763354No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC109920.1942464045329816No Hit
CCGACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGTCACC109810.19405201675551956No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG107180.18940438171256338No Hit
CCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCC106750.18864450221884813No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCG106710.18857381575431648No Hit
CCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAA105030.18560498424398705No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC100280.17721096658085328No Hit
AACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTT97000.1714146764892578No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC93170.16464644751035204No Hit
GGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC92880.16413397064249755No Hit
GGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGG90270.1595216788318072No Hit
TGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAG87240.15416717914353453No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACT85810.15164013803652793No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG85770.15156945157199628No Hit
TCCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATC84760.14978461834257206No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCC81780.14451847673496393No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC80220.14176170461822946No Hit
CCACAGGTCACGTCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGCATCC79460.14041866179212809No Hit
CCACCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCCACAGGTGACACC75500.13342070180349447No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC74700.1320069725128614No Hit
CACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCCA72190.1275713968635002No Hit
CCACAGGTCACACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC70610.12477928151449992No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT69510.12283540373987946No Hit
CAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAA69040.12200483778163254No Hit
CCACAGGTCAGGCCATCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCC67750.11972519930048675No Hit
CGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAG64900.11468878870260649No Hit
CACCACGCCTCTTCCTGTCACCATCCCTTCCTCAGCATCCTCAGGTCACA62770.11092473446629598No Hit
CACCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCCACAGGTGACACCA62300.11009416850804907No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGTATCC62260.1100234820435174No Hit
CAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGA61190.10813261911729571No Hit
CTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACCGACACT60970.10774384356237161No Hit
AGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTT59430.10502241467790299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)