FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003490711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052270
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC40280.38279148887642905No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT30520.2900396286124284No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC28430.27017780607638725No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC26400.2508861794026248No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC24980.23739154399536241No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT23800.2261776920372148No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC23530.22361181065696065No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT22890.21752972145932126No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC22650.2152489380102065No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT22460.21344331777965733No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC21690.20612580421374743No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG21210.2015642373155179No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG20410.19396162581846865No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA20220.19215600558791945No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA19700.18721430811483744No Hit
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG19620.1864540469651325No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC19370.18407823087230463No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT19340.18379313294116528No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC18480.17562032558183738No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT17980.1708686933961816No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA17910.17020346489018978No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT17290.1643114409799766No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC17180.16326608189913236No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT16670.15841941706976345No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC16350.15537837247094377No Hit
CCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGT15670.1489161526984519No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT14930.14188373706368138No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC14860.14121850855768955No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC14780.14045824740798465No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA14580.13855759453372235No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT14050.13352086441692723No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC13700.13019472188696818No Hit
AGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAA13680.13000465659954194No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC13520.1284841343001321No Hit
GGAAAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGG13480.12810400372527964No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG13280.12620335085101733No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC13060.12411263268932878No Hit
AGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG13040.12392256740190255No Hit
CCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTG12910.12268714303363204No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT12830.12192688188392713No Hit
CCTGTCCCTGGCCGACAAGGATGTGACTGCCAAAGACAGCAAGTTCAAAA12670.12040635958451729No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG12530.11907590257253366No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC11930.11337394394974674No Hit
ACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACT11840.1125186501563287No Hit
GTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCC11790.11204348693776313No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC11780.11194845429405001No Hit
CTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGA11670.11090309521320574No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT11490.10919250762636966No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT11320.1075769526832467No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG11200.10643656095868932No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG10750.10216009199159912No Hit
AGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT10700.10168492877303355No Hit
AGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTG10610.1008296349796155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)