FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003490715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950838
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG41640.2134467341726991No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC38440.1970435269356041No Hit
CACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCCA38380.19673596679990854No Hit
CCACAGGTCACGCCACCCCTCTTCATGTCACCAGCCCTTCCTCAGCATCC37340.19140492444785268No Hit
CACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACGCCA36820.18873940327182473No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC36040.18474112150778282No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC35680.18289576069360963No Hit
CCACAGGTCACGCCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC34990.179358819133111No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC33590.1721824159668819No Hit
CGACAAGGTGCGGTTCCTGGAGCAGCAGAACAAGGTTCTGGAAACAAAGT33060.16946563476823806No Hit
CACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG30710.15741952945349638No Hit
CCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCC28210.14460452379951588No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC28200.14455326377689998No Hit
CCGACACTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACC27630.1416314424877924No Hit
CCACAGGTCACGCCACCCCTCTTCTTGTCACCGACACTTCCTCAGCATCC26320.13491637952510663No Hit
CCACAGGTCAGGCCATCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCC24660.1264072157708636No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACT23250.11917955258201861No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC22960.11769301192615686No Hit
CCGACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGTCACC22810.11692411158691803No Hit
CCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTG22350.11456615054658563No Hit
CCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCGGTTCCTGGAGC22280.11420733038827416No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT21050.10790234760651576No Hit
CTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCT21010.10769730751605208No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC20930.10728722733512469No Hit
CGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG20810.10667210706373363No Hit
CTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACCGACACT20250.10380154579724199No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC20220.10364776572939424No Hit
CACCTCTCTTCCTGTCACCGACGCTTCCTCAGTGTCCACAGGTCACGCCA20190.10349398566154647No Hit
CCACAGGTCACGCCACCTCTCTTCTTGTCACCGACGCTTCCTCAGTATCC20110.1030839054806191No Hit
CACCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCCACAGGTGACACCA19950.10226374511876435No Hit
CTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGA19800.10149484477952551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)