FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003490718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067719
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG74480.24278625258701989No Hit
GTCGGTGACAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT67110.21876188790433543No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG65980.21507836930305546No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA64200.20927601256829587No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA63160.20588587155472843No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCTGAGGAAGGGC61560.20067026999539397No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT58070.18929373909409564No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGAGGTGG57500.18743568103858274No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT55730.18166592181356897No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGGTGG55670.18147033675509394No Hit
CTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCT51940.16931146561989543No Hit
GTCGGTGACAGGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT51820.16892029550294535No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT50820.1656605445283613No Hit
GTGTGACCTGAGGATGCTGAGGAAGGGCTGGTGACAGGAAGAGGGGTGGT50370.16419365658979848No Hit
CTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTGTC48680.15868467744275144No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT47940.15627246172155923No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG44750.14587385611263612No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA44710.14574346607365277No Hit
GCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTGT43730.1425489101185604No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC43590.14209254498211862No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA42640.1389957815562638No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGAGGATGCTGAGGAAGGGC41070.13387797252616684No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC40920.13338900987997923No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT40760.13286744972404577No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG39990.13035744147361605No Hit
CTGAGGAAGTGTCGGTGACAGGAAGAGAGGTGGCGTGACCTGTGGATGCT38810.12651093532360688No Hit
GTCGGTGACAAGAAGAGGGATGGCGTGACCTGTGGATGCTGAGGAAGGGC38040.12400092707317717No Hit
GTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGT37970.12377274450495629No Hit
CAGGAAGAGAGGTGGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACA37510.12227325905664761No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCTGAGGAAAGGC37250.12142572380325577No Hit
CAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACA36990.12057818854986392No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT34500.11246140862314964No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG33710.10988620535322825No Hit
GGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTG33620.10959282776551568No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC33150.10806074480746118No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGTAGATGCTGAGGAAGGGC32340.1054203465180481No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCC31450.1025191681506683No Hit
CTGAGGAAGTGTCGGTGACAAGAAGAGGGATGGCGTGACCTGTGGATGCT31160.10157384036803892No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC30780.10033513499769699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)