FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003490720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575311
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG120680.46860359777906435No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA113400.44033516728659183No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG92080.3575490494157793No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT91880.3567724441824696No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG84760.3291252978766448No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT72290.2807039615797859No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA71250.2766656143665755No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC71030.2758113486099349No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG68860.2673851818285248No Hit
CCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGA67520.2621819267653499No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC66130.2567845203938476No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA64950.2522025495173204No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG55780.21659519957007134No Hit
GCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGG53390.2073147670320206No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT53200.2065769920603764No Hit
GGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATC49790.19333587283244627No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT48140.18692887965764135No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC46730.18145381276280806No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC45520.17675535110128449No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC45080.17504681958800317No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG41810.1623493240233898No Hit
GGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGC40310.15652478477356715No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT40100.15570934927859198No Hit
CTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGT40080.155631688755261No Hit
AGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGA39210.15225345599036388No Hit
GTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGT38820.15073907578541No Hit
AGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCA36760.14274004188232023No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT33670.1307414910276856No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG33570.13035318841103075No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA33400.1296930739627175No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG32650.12678080433780617No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA32560.12643133198281684No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC31530.12243181503127196No Hit
CGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTG31100.12076211377965614No Hit
CTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTA30810.11963603619135707No Hit
CCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTG30770.11948071514469516No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC29470.1144327811281822No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC29070.11287957066156282No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG28490.11062741548496471No Hit
CGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTT28300.10988964051332052No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT28200.10950133789666568No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC27640.10732684324339856No Hit
AGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGAT27200.10561831173011725No Hit
GGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAG27170.10550182094512081No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG27050.105035857805135No Hit
CAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGT26970.1047252157118111No Hit
CGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGC26940.10460872492681467No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG26390.10247306053521303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)